Discover and publish cutting edge, open research.

Browse 71,094 multi-disciplinary research preprints

Featured documents

Michael Weekes

and 11 more

Nick K. Jones1,2*, Lucy Rivett1,2*, Chris Workman3, Mark Ferris3, Ashley Shaw1, Cambridge COVID-19 Collaboration1,4, Paul J. Lehner1,4, Rob Howes5, Giles Wright3, Nicholas J. Matheson1,4,6¶, Michael P. Weekes1,7¶1 Cambridge University NHS Hospitals Foundation Trust, Cambridge, UK2 Clinical Microbiology & Public Health Laboratory, Public Health England, Cambridge, UK3 Occupational Health and Wellbeing, Cambridge Biomedical Campus, Cambridge, UK4 Cambridge Institute of Therapeutic Immunology & Infectious Disease, University of Cambridge, Cambridge, UK5 Cambridge COVID-19 Testing Centre and AstraZeneca, Anne Mclaren Building, Cambridge, UK6 NHS Blood and Transplant, Cambridge, UK7 Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK*Joint first authorship¶Joint last authorshipCorrespondence: [email protected] UK has initiated mass COVID-19 immunisation, with healthcare workers (HCWs) given early priority because of the potential for workplace exposure and risk of onward transmission to patients. The UK’s Joint Committee on Vaccination and Immunisation has recommended maximising the number of people vaccinated with first doses at the expense of early booster vaccinations, based on single dose efficacy against symptomatic COVID-19 disease.1-3At the time of writing, three COVID-19 vaccines have been granted emergency use authorisation in the UK, including the BNT162b2 mRNA COVID-19 vaccine (Pfizer-BioNTech). A vital outstanding question is whether this vaccine prevents or promotes asymptomatic SARS-CoV-2 infection, rather than symptomatic COVID-19 disease, because sub-clinical infection following vaccination could continue to drive transmission. This is especially important because many UK HCWs have received this vaccine, and nosocomial COVID-19 infection has been a persistent problem.Through the implementation of a 24 h-turnaround PCR-based comprehensive HCW screening programme at Cambridge University Hospitals NHS Foundation Trust (CUHNFT), we previously demonstrated the frequent presence of pauci- and asymptomatic infection amongst HCWs during the UK’s first wave of the COVID-19 pandemic.4 Here, we evaluate the effect of first-dose BNT162b2 vaccination on test positivity rates and cycle threshold (Ct) values in the asymptomatic arm of our programme, which now offers weekly screening to all staff.Vaccination of HCWs at CUHNFT began on 8th December 2020, with mass vaccination from 8th January 2021. Here, we analyse data from the two weeks spanning 18thto 31st January 2021, during which: (a) the prevalence of COVID-19 amongst HCWs remained approximately constant; and (b) we screened comparable numbers of vaccinated and unvaccinated HCWs. Over this period, 4,408 (week 1) and 4,411 (week 2) PCR tests were performed from individuals reporting well to work. We stratified HCWs <12 days or > 12 days post-vaccination because this was the point at which protection against symptomatic infection began to appear in phase III clinical trial.226/3,252 (0·80%) tests from unvaccinated HCWs were positive (Ct<36), compared to 13/3,535 (0·37%) from HCWs <12 days post-vaccination and 4/1,989 (0·20%) tests from HCWs ≥12 days post-vaccination (p=0·023 and p=0·004, respectively; Fisher’s exact test, Figure). This suggests a four-fold decrease in the risk of asymptomatic SARS-CoV-2 infection amongst HCWs ≥12 days post-vaccination, compared to unvaccinated HCWs, with an intermediate effect amongst HCWs <12 days post-vaccination.A marked reduction in infections was also seen when analyses were repeated with: (a) inclusion of HCWs testing positive through both the symptomatic and asymptomatic arms of the programme (56/3,282 (1·71%) unvaccinated vs 8/1,997 (0·40%) ≥12 days post-vaccination, 4·3-fold reduction, p=0·00001); (b) inclusion of PCR tests which were positive at the limit of detection (Ct>36, 42/3,268 (1·29%) vs 15/2,000 (0·75%), 1·7-fold reduction, p=0·075); and (c) extension of the period of analysis to include six weeks from December 28th to February 7th 2021 (113/14,083 (0·80%) vs 5/4,872 (0·10%), 7·8-fold reduction, p=1x10-9). In addition, the median Ct value of positive tests showed a non-significant trend towards increase between unvaccinated HCWs and HCWs > 12 days post-vaccination (23·3 to 30·3, Figure), suggesting that samples from vaccinated individuals had lower viral loads.We therefore provide real-world evidence for a high level of protection against asymptomatic SARS-CoV-2 infection after a single dose of BNT162b2 vaccine, at a time of predominant transmission of the UK COVID-19 variant of concern 202012/01 (lineage B.1.1.7), and amongst a population with a relatively low frequency of prior infection (7.2% antibody positive).5This work was funded by a Wellcome Senior Clinical Research Fellowship to MPW (108070/Z/15/Z), a Wellcome Principal Research Fellowship to PJL (210688/Z/18/Z), and an MRC Clinician Scientist Fellowship (MR/P008801/1) and NHSBT workpackage (WPA15-02) to NJM. Funding was also received from Addenbrooke’s Charitable Trust and the Cambridge Biomedical Research Centre. We also acknowledge contributions from all staff at CUHNFT Occupational Health and Wellbeing and the Cambridge COVID-19 Testing Centre.

Guangming Wang

and 4 more

Tam Hunt

and 1 more

Tam Hunt [1], Jonathan SchoolerUniversity of California Santa Barbara Synchronization, harmonization, vibrations, or simply resonance in its most general sense seems to have an integral relationship with consciousness itself. One of the possible “neural correlates of consciousness” in mammalian brains is a combination of gamma, beta and theta synchrony. More broadly, we see similar kinds of resonance patterns in living and non-living structures of many types. What clues can resonance provide about the nature of consciousness more generally? This paper provides an overview of resonating structures in the fields of neuroscience, biology and physics and attempts to coalesce these data into a solution to what we see as the “easy part” of the Hard Problem, which is generally known as the “combination problem” or the “binding problem.” The combination problem asks: how do micro-conscious entities combine into a higher-level macro-consciousness? The proposed solution in the context of mammalian consciousness suggests that a shared resonance is what allows different parts of the brain to achieve a phase transition in the speed and bandwidth of information flows between the constituent parts. This phase transition allows for richer varieties of consciousness to arise, with the character and content of that consciousness in each moment determined by the particular set of constituent neurons. We also offer more general insights into the ontology of consciousness and suggest that consciousness manifests as a relatively smooth continuum of increasing richness in all physical processes, distinguishing our view from emergentist materialism. We refer to this approach as a (general) resonance theory of consciousness and offer some responses to Chalmers’ questions about the different kinds of “combination problem.”  At the heart of the universe is a steady, insistent beat: the sound of cycles in sync…. [T]hese feats of synchrony occur spontaneously, almost as if nature has an eerie yearning for order. Steven Strogatz, Sync: How Order Emerges From Chaos in the Universe, Nature and Daily Life (2003) If you want to find the secrets of the universe, think in terms of energy, frequency and vibration.Nikola Tesla (1942) I.               Introduction Is there an “easy part” and a “hard part” to the Hard Problem of consciousness? In this paper, we suggest that there is. The harder part is arriving at a philosophical position with respect to the relationship of matter and mind. This paper is about the “easy part” of the Hard Problem but we address the “hard part” briefly in this introduction.  We have both arrived, after much deliberation, at the position of panpsychism or panexperientialism (all matter has at least some associated mind/experience and vice versa). This is the view that all things and processes have both mental and physical aspects. Matter and mind are two sides of the same coin.  Panpsychism is one of many possible approaches that addresses the “hard part” of the Hard Problem. We adopt this position for all the reasons various authors have listed (Chalmers 1996, Griffin 1997, Hunt 2011, Goff 2017). This first step is particularly powerful if we adopt the Whiteheadian version of panpsychism (Whitehead 1929).  Reaching a position on this fundamental question of how mind relates to matter must be based on a “weight of plausibility” approach, rather than on definitive evidence, because establishing definitive evidence with respect to the presence of mind/experience is difficult. We must generally rely on examining various “behavioral correlates of consciousness” in judging whether entities other than ourselves are conscious – even with respect to other humans—since the only consciousness we can know with certainty is our own. Positing that matter and mind are two sides of the same coin explains the problem of consciousness insofar as it avoids the problems of emergence because under this approach consciousness doesn’t emerge. Consciousness is, rather, always present, at some level, even in the simplest of processes, but it “complexifies” as matter complexifies, and vice versa. Consciousness starts very simple and becomes more complex and rich under the right conditions, which in our proposed framework rely on resonance mechanisms. Matter and mind are two sides of the coin. Neither is primary; they are coequal.  We acknowledge the challenges of adopting this perspective, but encourage readers to consider the many compelling reasons to consider it that are reviewed elsewhere (Chalmers 1996, Griffin 1998, Hunt 2011, Goff 2017, Schooler, Schooler, & Hunt, 2011; Schooler, 2015).  Taking a position on the overarching ontology is the first step in addressing the Hard Problem. But this leads to the related questions: at what level of organization does consciousness reside in any particular process? Is a rock conscious? A chair? An ant? A bacterium? Or are only the smaller constituents, such as atoms or molecules, of these entities conscious? And if there is some degree of consciousness even in atoms and molecules, as panpsychism suggests (albeit of a very rudimentary nature, an important point to remember), how do these micro-conscious entities combine into the higher-level and obvious consciousness we witness in entities like humans and other mammals?  This set of questions is known as the “combination problem,” another now-classic problem in the philosophy of mind, and is what we describe here as the “easy part” of the Hard Problem. Our characterization of this part of the problem as “easy”[2] is, of course, more than a little tongue in cheek. The authors have discussed frequently with each other what part of the Hard Problem should be labeled the easier part and which the harder part. Regardless of the labels we choose, however, this paper focuses on our suggested solution to the combination problem.  Various solutions to the combination problem have been proposed but none have gained widespread acceptance. This paper further elaborates a proposed solution to the combination problem that we first described in Hunt 2011 and Schooler, Hunt, and Schooler 2011. The proposed solution rests on the idea of resonance, a shared vibratory frequency, which can also be called synchrony or field coherence. We will generally use resonance and “sync,” short for synchrony, interchangeably in this paper. We describe the approach as a general resonance theory of consciousness or just “general resonance theory” (GRT). GRT is a field theory of consciousness wherein the various specific fields associated with matter and energy are the seat of conscious awareness.  A summary of our approach appears in Appendix 1.  All things in our universe are constantly in motion, in process. Even objects that appear to be stationary are in fact vibrating, oscillating, resonating, at specific frequencies. So all things are actually processes. Resonance is a specific type of motion, characterized by synchronized oscillation between two states.  An interesting phenomenon occurs when different vibrating processes come into proximity: they will often start vibrating together at the same frequency. They “sync up,” sometimes in ways that can seem mysterious, and allow for richer and faster information and energy flows (Figure 1 offers a schematic). Examining this phenomenon leads to potentially deep insights about the nature of consciousness in both the human/mammalian context but also at a deeper ontological level.

Susanne Schilling*^

and 9 more

Jessica mead

and 6 more

The construct of wellbeing has been criticised as a neoliberal construction of western individualism that ignores wider systemic issues including increasing burden of chronic disease, widening inequality, concerns over environmental degradation and anthropogenic climate change. While these criticisms overlook recent developments, there remains a need for biopsychosocial models that extend theoretical grounding beyond individual wellbeing, incorporating overlapping contextual issues relating to community and environment. Our first GENIAL model \cite{Kemp_2017} provided a more expansive view of pathways to longevity in the context of individual health and wellbeing, emphasising bidirectional links to positive social ties and the impact of sociocultural factors. In this paper, we build on these ideas and propose GENIAL 2.0, focusing on intersecting individual-community-environmental contributions to health and wellbeing, and laying an evidence-based, theoretical framework on which future research and innovative therapeutic innovations could be based. We suggest that our transdisciplinary model of wellbeing - focusing on individual, community and environmental contributions to personal wellbeing - will help to move the research field forward. In reconceptualising wellbeing, GENIAL 2.0 bridges the gap between psychological science and population health health systems, and presents opportunities for enhancing the health and wellbeing of people living with chronic conditions. Implications for future generations including the very survival of our species are discussed.  

Mark Ferris

and 14 more

IntroductionConsistent with World Health Organization (WHO) advice [1], UK Infection Protection Control guidance recommends that healthcare workers (HCWs) caring for patients with coronavirus disease 2019 (COVID-19) should use fluid resistant surgical masks type IIR (FRSMs) as respiratory protective equipment (RPE), unless aerosol generating procedures (AGPs) are being undertaken or are likely, when a filtering face piece 3 (FFP3) respirator should be used [2]. In a recent update, an FFP3 respirator is recommended if “an unacceptable risk of transmission remains following rigorous application of the hierarchy of control” [3]. Conversely, guidance from the Centers for Disease Control and Prevention (CDC) recommends that HCWs caring for patients with COVID-19 should use an N95 or higher level respirator [4]. WHO guidance suggests that a respirator, such as FFP3, may be used for HCWs in the absence of AGPs if availability or cost is not an issue [1].A recent systematic review undertaken for PHE concluded that: “patients with SARS-CoV-2 infection who are breathing, talking or coughing generate both respiratory droplets and aerosols, but FRSM (and where required, eye protection) are considered to provide adequate staff protection” [5]. Nevertheless, FFP3 respirators are more effective in preventing aerosol transmission than FRSMs, and observational data suggests that they may improve protection for HCWs [6]. It has therefore been suggested that respirators should be considered as a means of affording the best available protection [7], and some organisations have decided to provide FFP3 (or equivalent) respirators to HCWs caring for COVID-19 patients, despite a lack of mandate from local or national guidelines [8].Data from the HCW testing programme at Cambridge University Hospitals NHS Foundation Trust (CUHNFT) during the first wave of the UK severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic indicated a higher incidence of infection amongst HCWs caring for patients with COVID-19, compared with those who did not [9]. Subsequent studies have confirmed this observation [10, 11]. This disparity persisted at CUHNFT in December 2020, despite control measures consistent with PHE guidance and audits indicating good compliance. The CUHNFT infection control committee therefore implemented a change of RPE for staff on “red” (COVID-19) wards from FRSMs to FFP3 respirators. In this study, we analyse the incidence of SARS-CoV-2 infection in HCWs before and after this transition.

How it works

Upload or create your research work
You can upload Word, PDF, LaTeX as well as data, code, Jupyter Notebooks, videos, and figures. Or start a document from scratch.
Disseminate your research rapidly
Post your work as a preprint. A Digital Object Identifier (DOI) makes your research citeable and discoverable immediately.
Get published in a refereed journal
Track the status of your paper as it goes through peer review. When published, it automatically links to the publisher version.
Learn More

Most recent documents

Natural selection was first proposed by Mr. Darwin and Mr. Wallace. Natural selection is a process that, during successive generations, leads to the creation of hereditary traits that increase the probability of survival and reproduction of living organisms in a population. In other words, natural selection selects alleles that have characteristics that help reproduction and survival. Alleles that increase the probability of survival and reproduction are passed on to the next generation. However, I want to argue that the heritable traits that increase the likelihood of survival, adaptation, and reproduction are created by natural selection based on certain fundamental principles. In other words, for living organisms to succeed in survival and adaptation, natural selection selects the hereditary characteristics of alleles based on certain fundamental principles. Fundamental principles: 1-energy and nutrient supply; 2-security supply; 3-adapting to environmental conditions such as temperature and humidity, etc.; 4-reproduction. These are the fundamental principles of natural selection that, if fulfilled, increase the probability of adaptation, survival and reproduction. Previously, these fundamental principles were briefly stated by Mr. Darwin and other biologists. However, the importance, value, and very important role of these fundamental principles in the process of natural selection and evolution have received very little attention. If we explain survival, adaptation, and natural selection without these fundamental principles, the concepts of survival, adaptation, and natural selection will not be correct and these basic and important concepts of biology will be incompletely and wrongly understood.

Haonan Jin

and 9 more

Objective: Postmenopausal women have a higher risk of developing Sjögren’s Disease (SjD) than men, indicating the involvement of sex hormones. The objective of this study was to investigate the causal relationship between sex hormones and SjD using a two-sample Mendelian randomization (MR) study. Methods: A two-sample MR analysis was conducted to examine sex hormone associations with SjD. Sex-stratified designs were employed, with additional databases used for further analysis of the outcomes. A Bonferroni correction was applied to ensure robust conclusions. Additionally, bioinformatics methods were employed to explore the underlying connections between sex hormones and SjD. Results: The results of the MR analysis showed a significant inverse association between BT levels and the risk of SjD, with each one standard deviation (SD) increase in BT levels associated with a 55% decrease in SjD risk ( P = 5.2357E–05). The sex-stratified analysis provided that, for every one SD increase in BT levels, the risk of SjD decreased by 27% in males and 20% in females ( P = 0.036, P = 0.0377). Specifically, among females, each one SD increase in TT levels resulted in a 28% reduction of SjD risk ( P = 0.0306). The bioinformatics analysis suggested that inflammation and immune-related pathways underlie their connection. Conclusion: Our study demonstrated a definitive causal relationship between androgen levels and the susceptibility to SjD, particularly among females. Androgen deficiency was shown to play a pivotal role in the pathogenesis of SjD, with inflammation and immune-related pathways underpinning the association.
Amyloid beta peptide (Aβ) aggregation in the brain represents an initial detrimental episode in the etiology of Alzheimer’s disease (AD). Recently, it has been discovered that inhibiting Aβ neurotoxicity by modulating highly toxic Aβ oligomers (AβOs) is more rewarding than reducing overall amyloid fibril production. In line with this, here we discuss the efficiency of multifunctional quinazolinone and vanillin acrylamide hybrids (QVA1-5) as modulators of aggregation behaviour. ThT assay inferred the dose dependent intensification of Aβ1-42 aggregation by QVA1-5. FE-SEM study disclosed enormously distinctive differences amongst the aggregate morphologies of Aβ1-42 and Aβ1-42 + QVA1-5 which intensely reinforced the modulatory action of QVA1-5 on the molecular assembly of Aβ1-42. Alamar Blue assay proved QVA1-5 as effective neuroprotectors in SH-SY5Y cell line against Aβ1-42 induced toxicity. Western blot showed increased Aβ1-42 fibrils in SH-SY5Y cells with QVA1-5. In molecular docking, all ligands had identical binding poses in sites 4-6 of Aβ fibril structure (PDB ID: 2M4J), where ligands span across five Aβ monomers that are stacked together and stabilized the fibril formation by hydrophobic interactions with the residues of Aβ monomers. In the molecular dynamic simulations, the lesser RMSD and similar rGyr values for ligands further supported the stability of the ligands inside the binding pocket of 2M4J Aβ fibril. All in all, the present study provides a mechanistic explanation at the atomic level for the impact of QVA1-5 on the Aβ fibril stabilization and shed light for the discovery of drugs that can manipulate the Aβ aggregates formation.

Tore Strohmeier

and 3 more

Aim: During the last decades mussels have shown a poleward shift in the distribution along the Atlantic coast conceivably driven by elevated sea-surface temperatures. We aimed to determine the baseline for the Northeast Atlantic (58 – 70° N) Mytilus species complex, and to compare the present distribution to surveys conducted 60 years ago. Location: Northeast Atlantic Methodology: Baseline was obtained by investigating a total of 509 stations in the intertidal zone, in four regions comprising the environmental gradient from head of fjord to coast, and distributed over the latitudinal gradient from 58 – 70° N. Results: The baseline shows a range in continuous abundance of mussels from 12 to 36 %, patchy abundance from 26 to 57 % and no or very limited mussel abundance from 26 to 46 % between the four regions. The presence of mussels in the southeast and west region was compared to previous surveys conducted 60 years ago. The data points to similar past and present presence of mussels in both regions, yet past major mussel fields in the inner section of region Southeast was not detected in this study. Main conclusions: The baseline of Mytilus spp. in the Northeast Atlantic (58 – 70° N) is now available for future reference. The baseline, compared to surveys conducted 60 years ago, points to awareness of the population situated in the southeast section of the investigated region. Continued monitoring and modelling are needed to clarify drivers of temporal and spatial variation in the mussel populations along the Northeastern Atlantic coast.

Feiyan Wang

and 2 more

Background Patients afflicted with rare non-malignant disorders endure the pain of the disease from birth. Receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) , especially umbilical cord blood transplantation (UCBT) , as soon as possible has becomes the only cure opportunity for such patients. For infants under six months old, the toxicity caused by chemotherapy-based conditioning regimens is a key issue that needs to be focused on. Nevertheless, consensus regarding specific dosage adjustments for conditioning regimens in such infants remains elusive. Objective Our pilot study aimed to determine a noval dose-adjusted conditioning regimen suitable for infants under 6 months of age with rare non malignant diseases who underwent unrelated umbilical cord blood transplantation. Methods We reported the transplant outcomes of five infants under 6 months old with rare non-malignant disease who underwent UCBT at our center from 2021 to 2024. Patients received a conditioning regimen containing adjusted dose of cyclophosphamide, fludarabine, anti-thymocyte immunoglobulin, busulfan and thiotepa, using≥8/10 UCB as the cell source. Results The median age of the patients was 4.2 months (range, 4.0 to 5.1) and the median weight of the patients was 5.5 kg (range, 3.5 to 7.5) at transplantation. These infants were diagnosed with Krabbe disease, very early onset inflammatory bowel disease (VEO-IBD), Shwachman-Diamond syndrome (SDS) and Methoxyvaleric aciduria. The median time for neutrophil engraftment and platelet engraftment in all patients was 24 days (range, 12-30 days) and 32 days (range, 26 to 46 days), respectively. The whole blood donor chimerism in all patients was>95% at 100 days post-UCBT, and all patients achieved a durable complete donor chimerism. The primary diseases of all patients have been effectively corrected, determined by regularly monitoring the enzyme concentration or gene mutation recovery. All patients survived until the follow-up date, and the event free survival rate after UCBT was 100%. Two out of five patients developed acute GVHD, but none had progressed to grade IV aGVHD or chronic GVHD. All patients achieved good lymphocyte reconstruction, especially with CD4+cells reaching over 200/uL in all patients at 100 days after UCBT. Moreover, natural killer cells and B cells also recover rapidly, with a immunoglobulin class switching. Conclusions: In summary, our preliminary research suggest that UCBT with this novel conditioning regimen offers a safe and effective option for infants under 6 months with rare non-malignant diseases, achieving early and rapid donor engraftment, good immune reconstitution with mild GVHD, and superior over survival.

Lucia Combrink

and 5 more

Adaptive radiations are rich laboratories for exploring, testing, and understanding key theories in evolution and ecology because they offer spectacular displays of speciation and ecological adaptation. Particular challenges to the study of adaptive radiation include high levels of species richness, rapid speciation, and gene flow between species. Over the last decade, high-throughput sequencing technologies and access to population genomic data have lessened these challenges by enabling the analysis of samples from many individual organisms at whole-genome scales. Here we review how population genomic data have facilitated our knowledge of adaptive radiation in five key areas: 1) phylogenetics, 2) hybridization, 3) timing and rates of diversification, 4) genomic basis of trait evolution, and 5) the role of genome structure in divergence. We review current knowledge in each area, highlight outstanding questions, and focus on methods that facilitate detection of complex patterns in population evolutionary demography. Among patterns that emerge, we particularly note that hybridization—and the hypothesized processes by which it shapes diversification—and questions seeking to bridge the divide between microevolutionary and macroevolutionary processes as rich areas for future study. Overall, access to population genomic data have facilitated an exciting era in adaptive radiation research, with implications for deeper understanding of fundamental evolutionary processes across the tree of life.

sergio gomez

and 6 more

Abstract Objective: The primary objective was to determine if consolidation with two short chemotherapy cycles using cytarabine plus idarubicin (AI) and high dose cytarabine plus mitoxantrone (haM) (two-cycle) reduced the cumulative incidence of relapse compared to the standard regimen of a 6-week consolidation phase (CONS) among newly diagnosed pediatric patients with acute myeloid leukemia (AML).   Methods:  GATLA 8-LMA-P’07 was a phase III trial conducted in 26 centers in Argentina. We included newly diagnosed pediatric patients with AML 0-18 years of age. Patients with M3 AML were excluded. After two cycles of induction, patients in remission were randomized to either CONS or two-cycle consolidation chemotherapy. High risk patients received matched family stem cell transplantation or maintenance therapy for 12 months.  Results: One hundred seven  patients younger than 18 years with de novo AML were randomized to CONS (n=52) or two-cycle (n=57). Cumulative incidence (standard error) of relapse was not significantly different between CONS (31% (0.1)) and two-cycle (39% (0.1)) consolidation (P=0.25). There was no significant difference in 5-year event free survival (53.6% (0.8) vs 44.3 (0.7), P= 0.31) or 5-year overall survival (55.0% (0.8) vs. 53.7% (0.7), P=0.91) for CONS and two-cycle consolidation respectively.  Conclusions: Consolidation with two cycles of chemotherapy was not significantly better than the standard consolidation in reducing the cumulative risk of relapse among newly diagnosed children with AML from Argentina. Future research should evaluate new approaches to improves outcomes for pediatric patients with AML.

Cory Bishop

and 5 more

The mutualism between the green alga Oophila amblystomatis ((Lambert, 1905 ex Wille, 1909) and egg masses of the spotted salamander Ambystoma maculatum (Shaw) involves acquisition of algal cells from water, followed by their population growth within embryonic capsules of the egg mass. Sampling thus far has been of insufficient geographic and sequencing scale to quantify the specificity of partner choice and whether it varies latitudinally. We used PacBio and Illumina sequencing of the 18S rRNA gene to investigate diversity in egg capsule fluid harvested from five locales separated by 1° latitude. We re-analyzed 18S (V4) and chloroplast 16S (V4/V5) reads from previous work using denoising protocols, which, along with current data, produced 16.37 million reads. We detected no chlorophytes outside the Oophila clade and detected cercozoan protists and rhizophydialean fungi in some capsules. There was a mean of 10.14  3.40 amplicon sequence variants (ASVs) per sample for Oophila, ranging in prevalence from endemic to omnipresent. We observed among-locale variation in mean ASVs/sample, but not in a latitudinal pattern. However, the mean proportion of the two most prevalent ASVs varied as a function of latitude. Pairwise alignments of ASVs from the V4 region revealed an inverse relationship between sequence identity and relative abundance in read counts. We conclude that the specificity of this mutualism is high, but additional closely related ASVs, with varying relative abundance and prevalence, are detectable. This mutualism may be suited as a model with which to test theories of partner choice.

Browse more recent preprints

Powerful features of Authorea

Under Review
Learn More
Journals connected to Under Review
Ecology and Evolution
Clinical Case Reports
Land Degradation & Development
Mathematical Methods in the Applied Sciences
Biotechnology Journal
Plant, Cell & Environment
International Journal of Quantum Chemistry
PROTEINS: Structure, Function, and Bioinformatics
All IET journals
All AGU journals
All Wiley journals
Featured Collection
Featured communities
Explore More Communities

Other benefits of Authorea


A repository for any field of research, from Anthropology to Zoology


Discuss your preprints with your collaborators and the scientific community

Interactive Figures

Not just PDFs. You can publish d3.js and graphs, data, code, Jupyter notebooks

Documents recently accepted in scholarly journals

Urbanization modifies ecosystem conditions and evolutionary processes. This includes air pollution, mostly as tropospheric ozone (O3), which contributes to the decline of urban and peri-urban forests. A notable case are fir(Abies religiosa) forests in the peripheral mountains southwest of Mexico City, which have been severely affected by O3 pollution since the 1970s. Interestingly, some young individuals exhibiting minimal O3—related damage have been observed within a zone of significant O3 exposure. Using this setting as a natural experiment, we compared asymptomatic and symptomatic individuals of similar age (≤15 years old; n = 10) using histological, metabolomic and transcriptomic approaches. Plants were sampled during days of high (170 ppb) and moderate (87 ppb) O3 concentration. Given that there have been reforestation efforts in the region, with plants from different source populations, we first confirmed that all analysed individuals clustered within the local genetic group when compared to a species-wide panel (Admixture analysis with ~1.5K SNPs). We observed thicker epidermis and more collapsed cells in the palisade parenchyma of needles from symptomatic individuals than from their asymptomatic counterparts, with differences increasing with needle age. Furthermore, symptomatic individuals exhibited lower concentrations of various terpenes (ß-pinene, ß-caryophylene oxide, α-caryophylene and ß-α-cubebene) than asymptomatic trees, as evidenced through GC-MS. Finally, transcriptomic analyses revealed differential expression for thirteen genes related to carbohydrate metabolism, plant defense, and gene regulation. Our results indicate a rapid and contrasting phenotypic response among trees, likely influenced by standing genetic variation and/or plastic mechanisms. They open the door to future evolutionary studies for understanding how O3 tolerance develops in urban environments, and how this knowledge could contribute to forest restoration.

Mohammad Rowshan

and 4 more

Channel coding plays a pivotal role in ensuring reliable communication over wireless channels. With the growing need for ultra-reliable communication in emerging wireless use cases, the significance of channel coding has amplified. Furthermore, minimizing decoding latency is crucial for critical-mission applications, while optimizing energy efficiency is paramount for mobile and the Internet of Things (IoT) communications. As the fifth generation (5G) of mobile communications is currently in operation and 5G-advanced is on the horizon, the objective of this paper is to assess prominent channel coding schemes in the context of recent advancements and the anticipated requirements for the sixth generation (6G). In this paper, after considering the potential impact of channel coding on key performance indicators (KPIs) of wireless networks, we review the evolution of mobile communication standards and the organizations involved in the standardization, from the first generation (1G) to the current 5G, highlighting the technologies integral to achieving targeted KPIs such as reliability, data rate, latency, energy efficiency, spectral efficiency, connection density, and traffic capacity. Following this, we delve into the anticipated requirements for potential use cases in 6G. The subsequent sections of the paper focus on a comprehensive review of three primary coding schemes utilized in past generations and their recent advancements: lowdensity parity-check (LDPC) codes, turbo codes (including convolutional codes), and polar codes (alongside Reed-Muller codes). Additionally, we examine alternative coding schemes like Fountain codes (also known as rate-less codes), sparse regression codes, among others. Our evaluation includes a comparative analysis of error correction performance and the performance of hardware implementation for these coding schemes, providing insights into their potential and suitability for the upcoming 6G era. Lastly, we will briefly explore considerations such as higher-order modulations and waveform design, examining their contributions to enhancing key performance indicators in conjunction with channel coding schemes.
The involvement of users in the product development process can significantly enhance product quality. The relationship between user experience and knowledge in product design contributes to product efficiency during the development phase. Users often struggle to align their perceptions, leading to extended product usage times and an inability to react to potential performance variations. Product manufacturers also face challenges in identifying suitable features that can positively impact product success and marketability. User experience in product interactions, encompassing both aesthetic and functional aspects, plays a pivotal role in influencing user evaluations and distinguishing characteristics crucial for achieving product success. Determining user knowledge’s influence on product success characteristics can provide valuable insights for the new product development process. This study conducted a survey to gather user experiences and knowledge, aiming to enhance the understanding of how users perceive products. This understanding is crucial for identifying product success characteristics, encompassing aspects such as specifications, sustainability, and recognition, which are instrumental in achieving overall product success. The results of the survey indicate that user knowledge, emotional experiences, and product attribute knowledge can assist product designers and manufacturers in identifying key characteristics for success during the early stages of the new product development process.

Browse more published preprints

Featured templates
Featured and interactive
Journals with direct submission
Explore All Templates