Nanoselenium enhanced wheat resistance to aphids by regulating
biosynthesis of DIMBOA and volatile components
Abstract
The mechanism of nanoselenium (nano-Se) improving plant components
induced resistance to aphids is unkonwn. . In this study, nano-Se (5.0
mg/L) foliar sprayed could significantly reduce Sitobion avenae number
(36%) compared with the control. Foliar application of nano-Se enhances
the antioxidant capacity by reducing MDA and increasing GSH-Px, CAT,
GSH, Pro and VE concentration in wheat seedlings. Phenylpropane pathway
was activated by nano-Se bio-fortification, which increased apigenin and
caffeic acid concentrations. The high-level expression of the related
genes (TaBx1A, TaBx3A, TaBx4A, TaASMT2, and TaCOMT) induced the
promotion of melatonin (88.6%) and DIMBOA (64.3%). Different ratios of
the secondary metabolites to nano-Se were conducted to examine the
effects on wheat resistance to the Sitobion avenae. The results revealed
the combination of nano-Se and melatonin can achieve the best overall
performance by reducing the Sitobion avenae number by 52.2%. The study
suggest that the coordinative applications of nano-Se and melatonin
could be more effectively improve the wheat resistance to aphids via
promotion of volatile organic compound synthesis and modulation in
phenylpropane and indole metabolism pathways.