2.8 Detection of volatile components in wheat seedlings.
Compared with other groups, the combination of nano-Se (5mg/L) and MT
(10mg/L) showed a better effect on reducing of aphid number. Gas
Chromatography-Ion Mobility Spectrometer (GC-IMS) was used to analyze
VOCs released from the wheat seedlings. About 0.5g wheat seedlings
samples were weighted in a 20-mL headspace vial. The vials were
equilibrated at 60℃ for 20 min before headspace injection. Table S4 (the
analysis conditions of GC-IMS unit) and Table S5 (gas chromatographic
conditions) have specific parameters. Wheat seedlings samples were
analyzed used with VOCAL and three plugins (Reporter, Gallery Plot, and
Dynamic PCA), which can qualitative and quantitative the data and
spectrogram.