Estimating the effect of two graph dextran-stearic acid-spermine
polymers based on magnetic iron oxide nanoparticles as carrier for gene
delivery
Abstract
Non-viral gene carriers because of their limited side effects,
biocompatibility, simplicity and taking the advantages of electrostatic
interactions have shown noticeable potential in gene delivery. The low
transfection rate of non-viral vectors under physiological conditions is
a significant issue. Here, the aim of this study was to investigate the
efficacy of hydrophilic and hydrophobic groups on gene carriers such as
two synthesized amphiphilic polymer of dextran-stearic acid-spermine
(DSASP) with verified lipid and amine conjugations that associated with
Fe3O4 superparamagnetic nanoparticles to promote the target delivery and
decrease the transfection time using static magnetic field. Our findings
illustrate that magnetic nanoparticles are spherical with positive
surface charges and superparamagnetic behaviors. The DSASP–pDNA/MNPs
offered a strong pDNA condensation, protection against DNase
degradation, significant cell viability in HEK 293T cells and. Although
conjugations of spermine play a critical role in transfection
efficiency, amphiphilic polymer with more derivatives of stearic acid
showed better transfection yields. Therefore, DSASP amphiphilic magnetic
carriers offer new insights for gene delivery due to the amine contents
and ameliorate the uptake of complexes via cell membrane based on its
hydrophilic surface.