Figure legends

Figure 1. The overview of amphiphilic polymer of dextran-stearic acid-spermine (DSASP) used to transfer nucleic acid via Fe3O4 encapsulation in the magnetofection process.
Figure 2. The (a, b) size and (c, d) zeta potential of Fe3O4 nanoparticle (MNPs) and dextran-stearic acid-spermine (DSASP)-pDNA/MNPs complexes were evaluated at different mass ratios.
Figure 3. Charactriaztion of synthesised MNPS. The SEM images of (a) Fe3O4 MNPs and(b) dextran-stearic acid-spermine (DSASP)@Fe3O4. The vibrating sample magnetometer (VSM) shows superparamagnetic property of MNPs based on no hysteresis loop, Hc = 0 Oe (c)Fe3O4 nanoparticles and (d)DSASP-Fe3O4 nanocomplexes. (e)FTIR spectra of Fe3O4 nanoparticles (red peak) DSASP polymer (blue peak) and the magnetic nanocomplex of DSASP–Fe3O4 (green peak).
Figure 4. Gel retardation analysis of dextran-stearic acid-spermine (DSASP) on pDNA by electrostatic interaction efficacy on 1% agarose gel at different mass ratios 1, 2.5, 5, 10, 20, 25, 50, 100 w/w of (a) DSA8SP-pDNA/MNPs and (b)DSA6SP-pDNA/MNPs. Serum stability assay with DNaseI in mass ratio of 50 w/w indicates for (c)DSA8SP-pDNA/MNPs and (d)DSA6SP-pDNA/MNPs.
Figure 5. The effect of different mass ratios (w/w) of triplex complexes of (a) pDNA-dextran-stearic acid 8-spermine (DS8ASP)-MNPs and (b) dextran–stearic acid 6–spermine (DS6ASP) pDNA/MNP and (c)combination treatments with MNPs based on the mass ratio of 10 w/w in presence and absence of SMF 20 mT on viability of HEK 293T cells. Data are shown as the mean ± SD based on independent tests (n = 4). **p < 0.01; ***p < 0.001 show significant differences relative to control (CTRL), which were analyzed by one-way factorial ANOVA followed with post-hoc Newman–Keuls multiple comparison tests.
Figure 6. Evaluation the transfection efficiency of(a) dextran-stearic acid 8-spermine (DS8ASP) and (b) dextran-stearic acid 6-spermine (DS6ASP) polyamines at different mass ratios in triplex complexes based on magnetofection processes. Data are shown as the mean ± SD based on independent tests (n = 4). *p < 0.05; **p < 0.01; ***p < 0.001 show significant differences relative to cells in absence of SMF, which were analyzed by one-way factorial ANOVA followed with post-hoc Newman–Keuls multiple comparison tests.