Figure legends
Figure 1. The overview of amphiphilic polymer of
dextran-stearic acid-spermine (DSASP) used to transfer nucleic acid via
Fe3O4 encapsulation in the
magnetofection process.
Figure 2. The (a, b) size and (c, d) zeta
potential of Fe3O4 nanoparticle (MNPs)
and dextran-stearic acid-spermine (DSASP)-pDNA/MNPs complexes were
evaluated at different mass ratios.
Figure 3. Charactriaztion of synthesised MNPS. The SEM images
of (a) Fe3O4 MNPs and(b) dextran-stearic acid-spermine
(DSASP)@Fe3O4. The vibrating sample
magnetometer (VSM) shows superparamagnetic property of MNPs based on no
hysteresis loop, Hc = 0 Oe (c)Fe3O4 nanoparticles and (d)DSASP-Fe3O4 nanocomplexes. (e)FTIR spectra of Fe3O4 nanoparticles (red
peak) DSASP polymer (blue peak) and the magnetic nanocomplex of
DSASP–Fe3O4 (green peak).
Figure 4. Gel retardation analysis of dextran-stearic
acid-spermine (DSASP) on pDNA by electrostatic interaction efficacy on
1% agarose gel at different mass ratios 1, 2.5, 5, 10, 20, 25, 50, 100
w/w of (a) DSA8SP-pDNA/MNPs and (b)DSA6SP-pDNA/MNPs. Serum stability assay with DNaseI in
mass ratio of 50 w/w indicates for (c)DSA8SP-pDNA/MNPs and (d)DSA6SP-pDNA/MNPs.
Figure 5. The effect of different mass ratios (w/w) of triplex
complexes of (a) pDNA-dextran-stearic acid 8-spermine
(DS8ASP)-MNPs and (b) dextran–stearic acid
6–spermine (DS6ASP) pDNA/MNP and (c)combination treatments with MNPs based on the mass ratio of 10 w/w in
presence and absence of SMF 20 mT on viability of HEK 293T cells. Data
are shown as the mean ± SD based on independent tests (n = 4). **p
< 0.01; ***p < 0.001 show significant differences
relative to control (CTRL), which were analyzed by one-way factorial
ANOVA followed with post-hoc Newman–Keuls multiple comparison tests.
Figure 6. Evaluation the transfection efficiency of(a) dextran-stearic acid 8-spermine (DS8ASP)
and (b) dextran-stearic acid 6-spermine
(DS6ASP) polyamines at different mass ratios in triplex
complexes based on magnetofection processes. Data are shown as the mean
± SD based on independent tests (n = 4). *p < 0.05; **p
< 0.01; ***p < 0.001 show significant differences
relative to cells in absence of SMF, which were analyzed by one-way
factorial ANOVA followed with post-hoc Newman–Keuls multiple comparison
tests.