Synergism Variation between Intracellular Glutathione, Phycocyanin and
SOD in Microalgae by Carbon Quantum Dot Fluorescence
Abstract
:Based on the use of CQDs as fluorescent probe and covalent coupling
method to detect biological molecules with amino groups, this method was
applied to the analysis and detection of metabolism of Microcystis
aeruginosa. The metabolic changes of carboxyl biomolecules in
Microcystis aeruginosa were analyzed by covalent coupling method,
including GSH, phycocyanin and SOD enzyme. The changes of GSH content in
Microcystis aeruginosa at different growth stages and the correlation
between phycocyanin, SOD and GSH were analyzed from one-dimensional,
two-dimensional and three-dimensional fluorescence. The content of
phycocyanin and SOD reached the maximum on the 65th day, and GSH was
more sensitive to the growth and metabolism of microalgae and began to
decrease on the 50th day. GSH plays an important role in reducing the
external oxidative damage of microalgae cells at each growth stage of
microalgae cells. Among them, GSH synthesis, GSH/GSSG mutual
transformation, synthesis of phytochelating peptides (PC), ASA-GSH cycle
and metabolism and other physiological activities in Microcystis
aeruginosa have interactions, which play a key role in maintaining the
antioxidant properties of microalgae and regulating redox-sensitive
signal transduction.