Shuyu Liu*, Xiaoyu Li , Zitong
School of Environment and Chemical Engineering, Shanghai University,
Shanghai, 201800, P. R. China
* Corresponding Author: Shuyu Liu
Email: liushuyu@shu. edu. cn
Ph: +86-13501947933
Fax: +86-021-6613787
Abstract :Based on the use of CQDs as fluorescent probe and
covalent coupling method to detect biological molecules with amino
groups, this method was applied to the analysis and detection of
metabolism of Microcystis aeruginosa. The metabolic changes of carboxyl
biomolecules in Microcystis aeruginosa were analyzed by covalent
coupling method, including GSH, phycocyanin and SOD enzyme. The changes
of GSH content in Microcystis aeruginosa at different growth stages and
the correlation between phycocyanin, SOD and GSH were analyzed from
one-dimensional, two-dimensional and three-dimensional fluorescence. The
content of phycocyanin and SOD reached the maximum on the 65th day, and
GSH was more sensitive to the growth and metabolism of microalgae and
began to decrease on the 50th day. GSH plays an important role in
reducing the external oxidative damage of microalgae cells at each
growth stage of microalgae cells. Among them, GSH synthesis, GSH/GSSG
mutual transformation, synthesis of phytochelating peptides (PC),
ASA-GSH cycle and metabolism and other physiological activities in
Microcystis aeruginosa have interactions, which play a key role in
maintaining the antioxidant properties of microalgae and regulating
redox-sensitive signal transduction.
Keywords: Metabolic mechanism; GSH; Phycocyanin ; SOD; CQD;