3.4 GSH metabolism and its correlation with phycocyanin, SOD
GSH is an important substance involved in the regulation of algal cell growth and metabolism, and as an important antioxidant in the process of algal cell growth and metabolism, it participates in the metabolism of the growth cycle in algal cells, and activates a variety of enzymes, thus promoting the metabolic activities of proteins and enzymes, etc., in algal cells. By analyzing the correlation between two substances, GSH and phycocyanin, the relationship between the methods of GSH and phycocyanin detection was obtained based on the relative signal values at different growth times: Y=0.4978X+0.0419, X indicates the relative signal value of GSH and Y indicates the relative signal value of phycocyanin, the maximum value of error is about 1.47 % and the minimum value is 0.46 %,. For the correlation between GSH and phycocyanin in algal cells during the growth phase, the correlation coefficient was 0.9925, which indicates a better correlation between GSH and phycocyanin, when the fluorescence change values of GSH and phycocyanin contents during the growth phase were then calculated.
By analyzing the correlation between the two substances of GSH and SODase, and comparing the relative signal values, the relationship between the methods of GSH and SODase detection was obtained as Y=0.2869X+0.0702, X indicates the relative signal value of GSH and Y indicates the relative signal value of SODase. The maximum value of error was about 1.78 %, while the minimum value was 0.09 %, and the correlation coefficient r was 0.9615, which means that the correlation between GSH and SOD enzyme in algal cells was good.GSH is a tripeptide with sulfhydryl group, which has an important role in reducing the external oxidative damage to microalgal cells. GSH plays a key role in the maintenance of antioxidant properties and the regulation of redox-sensitive signaling in microalgae, and the level of glutathione is associated with the resistance of microalgae to external stress. The level of GSH is closely related to the tolerance of microalgae cells to external stresses. The correlation suggests that as algal cells grow and increase in vital material, intracellular GSH subsequently increases to resist external oxidative slopes and environmental stresses.