3.4 GSH metabolism and its correlation with phycocyanin, SOD
GSH is an important substance involved in the regulation of algal cell
growth and metabolism, and as an important antioxidant in the process of
algal cell growth and metabolism, it participates in the metabolism of
the growth cycle in algal cells, and activates a variety of enzymes,
thus promoting the metabolic activities of proteins and enzymes, etc.,
in algal cells. By analyzing the correlation between two substances, GSH
and phycocyanin, the relationship between the methods of GSH and
phycocyanin detection was obtained based on the relative signal values
at different growth times: Y=0.4978X+0.0419, X indicates the relative
signal value of GSH and Y indicates the relative signal value of
phycocyanin, the maximum value of error is about 1.47 % and the minimum
value is 0.46 %,. For the correlation between GSH and phycocyanin in
algal cells during the growth phase, the correlation coefficient was
0.9925, which indicates a better correlation between GSH and
phycocyanin, when the fluorescence change values of GSH and phycocyanin
contents during the growth phase were then calculated.
By analyzing the correlation between the two substances of GSH and
SODase, and comparing the relative signal values, the relationship
between the methods of GSH and SODase detection was obtained as
Y=0.2869X+0.0702, X indicates the relative signal value of GSH and Y
indicates the relative signal value of SODase. The maximum value of
error was about 1.78 %, while the minimum value was 0.09 %, and the
correlation coefficient r was 0.9615, which means that the correlation
between GSH and SOD enzyme in algal cells was good.GSH is a tripeptide
with sulfhydryl group, which has an important role in reducing the
external oxidative damage to microalgal cells. GSH plays a key role in
the maintenance of antioxidant properties and the regulation of
redox-sensitive signaling in microalgae, and the level of glutathione is
associated with the resistance of microalgae to external stress. The
level of GSH is closely related to the tolerance of microalgae cells to
external stresses. The correlation suggests that as algal cells grow and
increase in vital material, intracellular GSH subsequently increases to
resist external oxidative slopes and environmental stresses.