BACKGROUND: Otomycosis is complicated for patients and otologists since it always needs long-term care and follow-up, but the recurrence rate remains high. Management is targeted toward intensive aural toileting with the administration of topical antifungals. Classical antifungal agents, including azoles and polyenes, have shown increased resistance. This has contributed to studies into medicinal plants as an alternative therapy for fungal infections. OBJECTIVE: This research aims to examine the potential vestibulotoxic effects of the henna leaf (Lawsomnia inermis), an ancient folk medicine, on the inner ear of a rat animal model. METHODS: Twenty-four healthy, adult male Wistar Kyoto rats were categorized into three groups (A, B, C) with eight rats each. Baseline vestibular parameters were tested before endoscopic-guided transtympanic instillation of aqueous and ethanol henna extract into the right middle ear of rats in Group A and B, respectively. At the same time, normal saline was instilled in the right middle ear of rats in Group C. Vestibular parameter testing was conducted on rats post-instillation of transtympanic Henna at intervals of 4 hours, 24 hours, 48 hours, 72 hours, one week, two weeks and three weeks. RESULTS: All vestibular parameters documented were not affected after the instillation of henna extract into the middle ear. CONCLUSION: Henna extract is a safe and potential alternative in treating otomycosis even with tympanic membrane perforation, as it has no vestibulotoxic adverse effects.