Until the isolation of IMNV from penaeid shrimps, the first Totiviridae member to be isolated from an arthopod, no Totiviridae member had been isolated from an arthopod, and more toti-like virus was discovered from arthopod. In this paper, we effectively isolated a novel totivirus-like particle from Culex tritaeniorhynchus designated NODE2. Sequence and phylogenetic analysis shows that the NODE2 has the similar genome organization just as some other members of Totiviridae family, and it may represent an entirely novel genus within the Totiviridae family.