Hydromorphodynamic interactions with vegetation are a part of fluvial biomorphodynamics in actively meandering rivers. Using palynofacies and grain size from sub-urban to urban reaches across the river valley, we examine the spatial patterns of organic matter behaviour in a 38 km reach of the river Gomati in Lucknow district, Uttar Pradesh, India, in an effort to understand how they responds to the alteration, preservation, and degradation after getting transported and deposited in sediment. To this we analyzed thirteen surface sediment samples of the Gomati River flood plain for palynofacies and grain size to ascertain its fate in this stretch which essentially is a big picture for past settlements. The shifts in the proportions of palynofacies associations viz. phytoclasts, palynomorphs, and amorphous organic matter (AOM) along with grain size aims to visualize the depositional process. The CONISS clusters analysis revealed four zones reflecting high degradation and alteration of palynofacies in the urban regime compared to the sub-urban reaches where the low interference with natural settings illustrate low deterioration of palynofacies. The relationship between grain size and palynofacies was obtained using Principal Component Analysis (PCA) to emphasize their correlation with palynofacies in this meandering fluvial system of Gomati River. In the flood plains deposits, the behaviours of palynofacies, allow for the distinction of the regional aspect of fluvial disposition. The study offers a comparison between urban and sub-urban settlement premises of today’s communities and contributes to our understanding of the growth, dispersal, and decline of earlier human settlements.