Gautam Ramesh

and 3 more

Background: Myocardial infarction (MI) often results in permanent cardiac tissue necrosis and reduced heart functionality. Even with heart disease being the number one cause of death in the US, there are currently no effective methods of fully regenerating heart muscle post-myocardial infarction. Mesenchymal stem cells (MSCs) are a promising therapeutic option given their multi-potent nature and low host immune reaction. Bioengineered polymeric nanofiber scaffolds provide a structured growing environment and encourage cell elongation. Overview: This study characterizes MSC biology on poly-ε-caprolactone (PCL) nanofiber scaffolds in order to establish electrospun PCL nanofibers as a working biological scaffold for MSC growth, and to exhibit potential for further exploration of PCL nanofiber-grown MSC implants as a treatment for MI. Methods: MSCs were seeded on PCL scaffolds; cell viability was analyzed via XTT, cell apoptosis was analyzed via TUNEL/DAPI staining, and differentiation markers were analyzed via RT-PCR. Results: TUNEL/DAPI staining of confluent MSCs on PCL scaffolds showed low cell apoptosis over time. RT-PCR results showed no amplification of CD40 expression. Conclusion: PCL nanofibers seem to provide a suitable microenvironment for MSC seeding and proliferation. Further Direction: RT-PCR for CD80, CD86, COL1A1 (collagen), aSMA/Acta2 (smooth muscle), Flk1/VEGF2 (endothelial), Sparc (Osteonectin, osteogenic), Adipoq (Adiponectin, adipogenic), Agre-cano (chondrogenic).

Gautam Ramesh

and 1 more

We, as students in the medical field, have the unique privilege of playing an influential role in transforming the system that we are trainees of. It is our prerogative to observe, internalize, scrutinize, and reform the medical curriculum to reflect what we believe will make the largest impact on our patients, and the patients of future students at our institution. Over the last twelve months, we have seen numerous efforts at medical schools across the nation to push forth a curriculum more mindful of health disparities and racism in medicine. Student-changemakers at our school have spearheaded a multi-year Health Equity curriculum, inspiring faculty to create coursework centered around educating students on social determinants of health, gender and race disparities in care, health outcomes based on age, incarceration, homelessness, and more.Our own impetus was borne of the realization that the healthcare system has devolved into a disease-care system. While the overwhelming majority of chronic disease can be altogether prevented through healthy lifestyle behaviors \cite{Ford2009}, few Americans engage in adequate physical activity, follow a healthy diet, maintain a healthy weight, and abstain from smoking \cite{Reeves2005}. These lifestyle deficiencies arise not from laziness; rather, numerous external factors induce these behaviors, such as proximity of grocers and famers markets, availability of safe recreation spaces, presence of community support, racial inequities, and influence of media and advertising. To educate our peers and the future medical students at our school, we created a seminar series and a health equity lecture to highlight the countless determinants at play when it comes to a patient’s health. By emphasizing the importance of lifestyle behaviors in preventing disease, and underscoring how to address these behaviors in a clinical encounter, we aim to erect a pillar of compassion in our curriculum, training future physicians to consider all potential influences on a patient’s health, and to advocate for them through the creation of wellness-centered policy. A gap in medical training is not just a lapse of understanding; it is an instant of substandard patient care that could be better – diseases that could be prevented, and lives that could be saved. By editing the next iteration of medical education, we, as medical students, hold the power to rewrite the future of the health of our country. ------------------------------------------------------------------------------------------------------------------------------------Acknowledgements: The authors thank Dr. Deepa Sannidhi, M.D., and Dr. Weena Joshi, M.D., for their guidance and mentorship in these endeavors. Funding/Support: None. Other disclosures: None. Ethical approval: Not applicable. Disclaimers: None.