Gentamicin (GM) is an aminoglycoside antibiotic with a rapid bactericidal effect in the treatment of many infections. However, its use in high concentrations for more than 7 days causes nephrotoxic side effects. It was aimed to determine the biochemical and molecular activities of resatorvid and alpha lipoic acid (ALA) on GM induced nephrotoxicity in rats. In the study, 34 Wistar albino rats were randomly distributed; Healthy control (n:6), Gentamicin (80 mg/kg, n:7), Gentamicin+Sham (%10 hydroalcoholic solution, n:7), Gentamicin+resatorvid (5 mg/kg, n:7), Gentamicin+ALA (100 mg/kg, n:7). Urinary IL-18, KIM-1 and NGAL levels were statistically decreased by resatorvid treatment, and KIM-1 levels were statistically decreased by ALA treatment compared to the gentamicin group. In addition, urine creatinine was partially decreased by resatorvid and ALA. NRF-2, CAS-3 and NR4A2 expressions were statistically decreased by resatorvid and ALA treatment. Nevertheless, NF-κB was statistically decreased only with resatorvid. The nephrotoxicity that limits the use of gentamicin in infections can reduce by resatorvid in the future. Therefore, it can be used for a longer and more effective in infections.