Comparing Haematoxylin-eosin with Papanicolaou Stain for Demonstration
of Endometrial Cytological Examination
Abstract
Objective To provide a better pathological method for clinical diagnosis
of endometrial lesions. Design Comparing the accuracy of
hematoxylin-eosin (H-E) with Papanicolaou stain for endometrial
cytology. Setting The First Affiliated Hospital of Xi’an Jiaotong
University. Population or Sample 180 patients. Methods Endometrial
slides were stained by H-E and Papanicolaou methods, respectively. The
cytology between them were compared, with histology as standard control,
as well as the cost performance. Main Outcome Measures The dyeing degree
of satisfaction, sensitivity, specificity, Cohen’s kappa coefficient,
false positive rate, false negative rate, positive likelihood ratio,
negative likelihood ratio, Youden index, positive predictive values, and
negative predictive values. The price and service time of dye, dyeing
efficiency, and dyeing time. Results The sensitivity of H-E stain was
higher than that of Papanicolaou stain. The specificity of H-E stain was
same to that of Papanicolaou stain. Cohen’s kappa coefficient indicatied
a high consistency between them. The morphological characterization of
Papanicolaou stain were superior to those of H-E stain. The cost
performance of H-E stain was lower. Conclusions In terms of overall
evaluations and economic benefits, H-E stain is better than Papanicolaou
stain. If a small sample volume of endometrial cells, Papanicolaou stain
is recommended. Funding The Clinical Research Award of the First
Affiliated Hospital of Xi’an Jiaotong University, China, the Major Basic
Research Project of Natural Science of Shaanxi Provincial Science and
Technology Department, the Key Research and Development Project of
Shaanxi Provincial Science and Technology Department. Keywords: H-E
stain, Papanicolaou stain, Endometrium, Cytology