Patient and Public Involvement
Aim: To provide endometria for comparing the accuracy between H-E and Papanicolaou stain.
Methods: Patients were enrolled in the First Affiliated Hospital of Xi’an Jiaotong University from October 2018 to July 2020. The inclusion criteria were as follows: patients with irregular uterine discharge of blood and fluid, or endometrial abnormality revealed by imaging tests, who underwent fractional dilatation and curettage, and patients who underwent hysterectomy. The exclusive diseases included acute severe systemic disease, cervical cancer, ovarian cancer, peritoneal cancer, pregnancy or suspected pregnancy, abnormal coagulation function and other hematological system diseases affecting coagulation, body temperature over 37.5 degrees Celsius twice on same day. The related histological diagnosis was controlled as a gold standard. The study was approved by the Institutional Review Board of the First Affiliated Hospital of Xi’an Jiaotong University (ChiCTR1800020123). The informed consents were obtained from all patients. All participants were numbered according to the orders of registration. Before fractional dilatation and curettage or hysterectomy, the endometrial cells of patients were sampled with Li brushes (Xi’an Meijiajia Bio-Technologies Co. Ltd., China) (23). The endometrial sample from same patient was made into two cytopathological sections through liquid based thin-layer cell method and then stained by H-E and Papanicolaou method, respectively (Xi’an Meijiajia Bio-Technologies Co. Ltd., China).
Study results: Some patient meeting the criterion rejected sampling. Total 180 patients were included in the study finally, who underwent fractional dilatation and curettage, or hysterectomy. Each method had samples with poor dyeing effect.
Discussion and conclusions: The public cooperation was important to the conduct of our experiment. Patients need to be communicated carefully and skillfully. The quantity and quality of samples would affect the study result.
Reflections/critical perspective: We should promote and propagandize the early screening and hazards of endometrial cancer well to increase the participation rate of patients. The communicated skill must be mastered by sampling persons.