ABSTRACT
Objective To provide a better pathological method for clinical
diagnosis of endometrial lesions.
Design Comparing the accuracy of hematoxylin-eosin (H-E) with
Papanicolaou stain for endometrial cytology.
Setting The First Affiliated Hospital of Xi’an Jiaotong
University.
Population or Sample 180 patients.
Methods Endometrial slides were stained by H-E and Papanicolaou
methods, respectively. The cytology between them were compared, with
histology as standard control, as well as the cost performance.
Main Outcome Measures The dyeing degree of satisfaction,
sensitivity, specificity, Cohen’s kappa coefficient, false positive
rate, false negative rate, positive likelihood ratio, negative
likelihood ratio, Youden index, positive predictive values, and negative
predictive values. The price and service time of dye, dyeing efficiency,
and dyeing time.
Results The sensitivity of H-E stain was higher than that of
Papanicolaou stain. The specificity of H-E stain was same to that of
Papanicolaou stain. Cohen’s kappa coefficient indicatied a high
consistency between them. The morphological characterization of
Papanicolaou stain were superior to those of H-E stain. The cost
performance of H-E stain was lower.
Conclusions In terms of overall evaluations and economic
benefits, H-E stain is better than Papanicolaou stain. If a small sample
volume of endometrial cells, Papanicolaou stain is recommended.
Funding The Clinical Research Award of the First Affiliated
Hospital of Xi’an Jiaotong University, China, the Major Basic Research
Project of Natural Science of Shaanxi Provincial Science and Technology
Department, the Key Research and Development Project of Shaanxi
Provincial Science and Technology Department.
Keywords: H-E stain, Papanicolaou stain, Endometrium, Cytology