Patient and Public Involvement
Aim: To provide endometria for comparing the accuracy between H-E and
Papanicolaou stain.
Methods: Patients were enrolled in the First Affiliated Hospital of
Xi’an Jiaotong University from October 2018 to July 2020. The inclusion
criteria were as follows: patients with irregular uterine discharge of
blood and fluid, or endometrial abnormality revealed by imaging tests,
who underwent fractional dilatation and curettage, and patients who
underwent hysterectomy. The exclusive diseases included acute severe
systemic disease, cervical cancer, ovarian cancer, peritoneal cancer,
pregnancy or suspected pregnancy, abnormal coagulation function and
other hematological system diseases affecting coagulation, body
temperature over 37.5 degrees Celsius twice on same day. The related
histological diagnosis was controlled as a gold standard. The study was
approved by the Institutional Review Board of the First Affiliated
Hospital of Xi’an Jiaotong University (ChiCTR1800020123). The informed
consents were obtained from all patients. All participants were numbered
according to the orders of registration. Before fractional dilatation
and curettage or hysterectomy, the endometrial cells of patients were
sampled with Li brushes (Xi’an Meijiajia Bio-Technologies Co. Ltd.,
China) (23). The endometrial sample from same patient was made
into two cytopathological sections through liquid based thin-layer cell
method and then stained by H-E and Papanicolaou method, respectively
(Xi’an Meijiajia Bio-Technologies Co. Ltd., China).
Study results: Some patient meeting the criterion rejected sampling.
Total 180 patients were included in the study finally, who underwent
fractional dilatation and curettage, or hysterectomy. Each method had
samples with poor dyeing effect.
Discussion and conclusions: The public cooperation was important to the
conduct of our experiment. Patients need to be communicated carefully
and skillfully. The quantity and quality of samples would affect the
study result.
Reflections/critical perspective: We should promote and propagandize the
early screening and hazards of endometrial cancer well to increase the
participation rate of patients. The communicated skill must be mastered
by sampling persons.