Abstract
This paper describes a new method for
preparation of chromogen free oxidase based optical biosensors. Oxidases enzymes were deposited on the
titania film consisting of uniformly distributed nanoparticles reacting with
hydrogen peroxide thus providing formation of yellow color due to complex Ti
(IV)-H2O2. Using ink jet printing and specially developed
titania ink we have created a universal platform for the oxidase based
biosensors and successfully tested it on glucose and cholesterol detection. The
paper fully discusses new biomaterials as well as biosensor creation technology
and describes the mechanism of action, study on stability and working
concentration limits and adjusting composition of the inks for good
printability and scale-up production.