This paper describes a new method for preparation of chromogen free oxidase based optical biosensors. Oxidases enzymes were deposited on the titania film consisting of uniformly distributed nanoparticles reacting with hydrogen peroxide thus providing formation of yellow color due to complex Ti (IV)-H₂O₂. Using ink jet printing and specially developed titania ink we have created a universal platform for the oxidase based biosensors and successfully tested it on glucose and cholesterol detection. The paper fully discusses new biomaterials as well as biosensor creation technology and describes the mechanism of action, study on stability and working concentration limits and adjusting composition of the inks for good printability and scale-up production.