Background: Measuring the nurses’ perception of justice in the performance appraisal system is important in achieving effective performance appraisal results. Therefore, this study aimed to evaluate the psychometrics of the Persian version of the Justice Perception of Performance Appraisal (JPPA)instrument. Methods: In this methodological study, 301 clinical nurses working in the hospitals affiliated to Shahid Beheshti University of Medical Sciences were selected based on purposive sampling. The translation and validation of the justice perception of the performance appraisal framework were sought according to the instructions proposed by the University of Incarnate Word in the United States. The psychometric characteristics of the instrument including construct validity (exploratory and confirmatory factor analysis) and reliability were evaluated using internal consistency based on Cronbach’s alpha index and intra-class correlation (ICC). In the present study, SPSS version 17 and LISREL software version 8.8 were used for confirmatory factor analysis. Results: The results of the exploratory factor analysis indicated the establishment of these four factors: procedural justice, distributive justice, interpersonal justice and informational justice which covered 57.71% of the total variance. The results of the confirmatory factor analysis confirmed the appropriateness of the four-factor construct of the justice perception of performance appraisal (p = 0.000, X2 = 4184.26, df = 1478, GFI = 0.67 & AGFI = 0.64, RMR = 0.11, SRMR = 0.078, &RMSEA = 0.078, NFI = 0.95& NNFI = 0.97, and CFI = 0.97). The internal consistency of the instrument was also confirmed according to the Cronbach’s alpha index of 0.97 and the intra-class correlation index of 0.95. Conclusion: The Persian version of the Justice Perception of Performance Appraisal can be used as a valid and reliable instrument to measure the level of nurses ‘perception of justice in the performance appraisal.