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Preparation of Chitosan Embedded Recombinant Human Epidermal Growth Factor Nanoparticles as Accelerating Compounds for Skin Remodeling in Chronic Lesions
  • saadeh hashemi,
  • Elnaz Mihandoost,,
  • Sepideh Khaleghi
saadeh hashemi
Islamic Azad University Tehran Medical Sciences

Corresponding Author:saadeh.hashemi@gmail.com

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Elnaz Mihandoost,
Islamic Azad University Tehran Medical Sciences
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Sepideh Khaleghi
Islamic Azad University
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Chronic lesion has become a major biological burden for individual patients and health organizations. Using nanoparticles as drug delivery systems is remarkable nowadays. The unique properties of chitosan without any toxicity for living creations make it a suitable option for drug delivery. Epidermal growth factor (EGF) is one of the important agents for wound healing, cellular proliferation, extracellular matrix formation, and skin remodeling. A combination of these properties can accelerate the wound healing process. In this study, rh-EGF is embedded into the chitosan nanoparticles by the Ion-gelation method. Nanoparticles are characterized by TEM microscopy and the DLS method and conjugation efficacy is measured by FT-IR radiation. The antibacterial effect of manipulated nanoparticles was estimated by MIC/MBC methods. The cytotoxicity and proliferation were measured by MTT assay on the HFF-1 human fibroblast cell line. Migration assay was accomplished by in vitro scratch model and the gene expression analysis for TGF-β, VEGF, and PDGF were manipulated by the real time-PCR method. The obtained results were considered statistically significant with P < 0.05. Obtained results illustrated no toxic effect on the HFF-1 cell line treated with Chitosan-EGF (CS-EGF). In cellular proliferation and migration assays, CS-EGF nanoparticles demonstrated a better effect than free rh-EGF. For the duration of 72h of the experiment, the whole scratch was covered by fibroblasts. The real time-PCR analysis also showed upregulation of all TGF-β, VEGF, and PDGF genes. As CS-EGF nanoparticles in the acceleration of the skin remodeling process showed promising results, subsequent studies might be useful.
15 Aug 2022Submitted to Micro & Nano Letters
05 Sep 2022Submission Checks Completed
05 Sep 2022Assigned to Editor
18 Sep 2022Reviewer(s) Assigned
31 Jan 2023Review(s) Completed, Editorial Evaluation Pending
01 Feb 2023Editorial Decision: Revise Major
28 Feb 20231st Revision Received
01 Mar 2023Assigned to Editor
01 Mar 2023Submission Checks Completed
09 Mar 2023Reviewer(s) Assigned
28 Apr 2023Review(s) Completed, Editorial Evaluation Pending
30 Apr 2023Editorial Decision: Revise Minor
30 Apr 20232nd Revision Received
01 May 2023Submission Checks Completed
01 May 2023Assigned to Editor
03 May 2023Reviewer(s) Assigned
12 Jun 2023Review(s) Completed, Editorial Evaluation Pending
12 Jun 2023Editorial Decision: Accept