In the present study, tin nanoparticles were green-synthesized using the aqueous extract of Foeniculum vulgare leaf aqueous extract. The synthesized SnNPs were characterized by analytical techniques including EDX, FE-SEM, XRD, UV-Vis., and FT-IR. The anti-human gastric cancer activity of SnNPs was evaluated using MTT assay. The nanoparticles were formed in a spherical shape in the range size of 26.45 to 38.53 nm. In the antioxidant test, the IC50 of F. vulgare, SnNPs@FV, and BHT against DPPH free radicals were 384, 119, and 71 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with SnNPs@FV were assessed by MTT assay for 48h about the cytotoxicity and anti-human lung cancer properties on normal (HUVEC) and lung cancer cell lines i.e., NCI-H2126, NCI-H1299, and NCI-H1437. The IC50 of SnNPs@FV were 108, 168, and 122 µg/mL against NCI-H2126, NCI-H1299, and NCI-H1437 cell lines, respectively. The viability of malignant lung cell line reduced dose-dependently in the presence of SnNPs@FV.
In this study, the Zn nanoparticles was synthesized using the peel extract of Citrus aurantium. The nanoparticles was characterized by different chemical technique including UV-Vis. and FT-IR spectroscopy, and SEM technique. The results revealed a spherical shape in the average size of 41.17 nm was identified for the green-synthesized nanoparticles. In the antioxidant test, the IC50 of nanoparticles and BHT against DPPH free radicals were 115 and 96 µg/mL, respectively. In the cellular and molecular part of the recent study, the treated cells with nanoparticles were assessed by MTT assay for 48h about the cytotoxicity and anti-human gastric cancer properties on normal (HUVEC) and gastric cancer cell lines i.e. NCI-N87 and MKN45. The IC50 of nanoparticles were 278 and 256 µg/mL against NCI-N87 and MKN45 cell lines, respectively. The viability of malignant gastric cell line reduced dose-dependently in the presence of Zn nanoparticles. It seems that the anti-human gastric cancer effect of recent nanoparticles is due to their antioxidant effects. After evaluating the effectiveness of this formulation in clinical trial researches, it can be a good alternative to chemotherapy drugs.
Tetracalcium phosphate (TTCP) is one of the main powder components in self-setting calcium phosphate cements for hard tissue applications. In this study, two types of calcium phosphate/chondroitin sulfate bone cements in which TTCP powders in nanoscale-rod like (R-TTCP) and micro-conventional irregular shape (C-TTCP) were used. The first one was synthesized by reverse microemulsion chemical process and the second one, was prepared by thermal conventional method. The results showed that both cements formed hydroxyapatite as the result of cementation process. The R-TTCP cement revealed a slightly longer initial but no difference in final setting time, less compressive strength, higher porosity and better degradation behavior compared to C-TTCP one. The both cements presented similar tendency to the formation of a dense hydroxyapatite on their outer surfaces through immersion in simulated body fluid. Taking into consideration the initial porosity, the cement made from R-TTCP rod like nanopowder presented more aptness to participate in ion exchange in SBF resulting to fill the 15% more initial porosity via the precipitation of hydroxyapatite mineral. From the biological point of view, analysis of cytotoxicity and MG63 osteoblastic-cell behavior proved that the both cements had good viability and proper cell adhesion and activity.