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Optimization of Chlorella vulgaris cultivation grown in waste molasses syrup using mixture design
  • Fahimeh Sadat Mohammadi,
  • Daryush Arabian
Fahimeh Sadat Mohammadi
Malek-Ashtar University of Technology

Corresponding Author:[email protected]

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Daryush Arabian
Malek Ashtar University of Technology - Shahin Shahr Campus
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The aim of this study was to determine and optimize culture media for Chlorella vulgaris microalgae under mixotrophic conditions using waste molasses as a cheap carbon source containing both organic carbons and other nutrients. In the current study, at first the growth and lipid productivity of Chlorella vulgaris were assessed in different culture media and the best media was selected for mixotrophic growth conditions. Significant medium ingredients were screened through Plackett–Burman design. Then ingredients with positive effect were considered as a mixture component and their combinations were evaluated on lipid productivity using mixture design. According to results, Zarrouk medium was considered as the base medium with the highest biomass and lipid productivity of 72 and 7.1 mg/l.d , respectively. Based on the Plackett–Burman design, out of eleven factors, molasses, NaNO3 and K2HPO4 demonstrated key roles in biomass and lipid productivity in mixotrophic conditions. Consequently, the selected three factors were investigated by mixture design. The results showed that high concentration of molasses causes decrease in biomass and lipid productivity due to high turbidity and a blend consisting of approximately 9.5 g/l molasses, 5 g/l NaNO3 and 0.15 g/l K2HPO4 was found as the optimum mixture with obtained lipid productivity of 115 mg/l.d. In conclusion, waste molasses can be used as a promising feedstock for cost effective cultivation of C. vulgaris.
12 Jul 2022Submitted to Journal of the American Oil Chemists' Society
12 Jul 2022Submission Checks Completed
12 Jul 2022Assigned to Editor
25 Jul 2022Reviewer(s) Assigned
20 Aug 2022Review(s) Completed, Editorial Evaluation Pending
20 Aug 2022Editorial Decision: Revise Major
08 Sep 20221st Revision Received
08 Sep 2022Submission Checks Completed
08 Sep 2022Assigned to Editor
19 Sep 2022Reviewer(s) Assigned
07 Oct 2022Review(s) Completed, Editorial Evaluation Pending
09 Oct 2022Editorial Decision: Accept
Jan 2023Published in Journal of the American Oil Chemists' Society volume 100 issue 1 on pages 45-56. 10.1002/aocs.12656