Enrichment of palmitoleic acid by a combination of crystallization and
Palmitoleic acid shows a variety of beneficial properties to human
health. In this study, enrichment of palmitoleic acid from sea buckthorn
pulp oil by crystallization and molecular distillation was investigated.
Sea buckthorn pulp oil was first converted to its corresponding mixed
fatty acids (SPOMFs) that contained 27.17% palmitoleic acid.
Subsequently, the effect of various factors on crystallization (i.e.,
crystallization temperature, solvent, ratio of SPOMFs to solvent (w/v),
crystallization time) and molecular distillation (distillation
temperature) were assessed on a 5-g scale. It was found that optimal
crystallization conditions were a 1:15 ratio of SPOMFs to methanol (w/v)
at -20 °C for 12 h, while the optimal temperature for molecular
distillation was 100 °C. These conditions were utilized to obtain a
liquid oil comprising 54.18% palmitoleic acid with an overall yield of
56.31%. This method has great potential for adoption by the food and
medical industries for the preparation of palmitoleic acid concentrate
for nutritional studies.