Single Cell Dynamics

Reference list:

Reference Year Model Method Aim
(Lackner et al., 2015) 2015 HAP1 CRISPR/Cas9 (TurboGFP) Endogenous gene expression
NanoLuc Subcellular localisation
(Xi et al., 2015) 2015 HEK-293 CRISPR/Cas9 (eGFP-Puro) Fluorescent microscopy
N-terminal FLAG-SNAP tag (TERT)
(Tanenbaum et al., 2014) 2014 HEK-293 Antibody-based (GFP) Fluorescent microscopy
CRISPR-Cas9: synthetic transc. factor Signal amplification
(Leonetti et al., 2016) 2016 HEK-293T CRISPR/Cas9 (Split GFP system) Proteome-wide analysis
GFP11
(Deng et al., 2015) 2015 MEFs, HeLa Nuclease-deficient CRiSPR/Cas9 Labeling sequence-specific
as probes loci fluorescently
(Kwon et al., 2015) 2015 Porcine parthenogenetic CRISPR/Cas9 Oct4 GFP Protein dynamics
zygotes Knock-in
(Ratz et al., 2015) 2015 U2OS CRISPR/Cas9 GFP Super resolution
Knock-in microscopy
(Chen et al., 2013) 2013 HEK-293T CRISPR/Cas9 Imaging genomic loci
eGFP-tagged endonuclease- in living human cells
deficient Cas9
(Stewart-Ornstein et al., 2016) 2016 MCF-7 CRISPR/Cas9 Protein dynamics in
eFLuT tagging system single cells
(Böttcher et al., 2014) 2014 D. melanogaster CRISPR/Cas9 Chromosomal gene
S2 cells GFP modification
(Chen et al., 2016) 2015 HEK-293T, RPE CRISPR/Cas9 Tagging multiple loci
Telomere, MUC4E
(Issaeva et al., 2010) 2010 H1299 Directed Central Dogma Generating reporter
tagging - artificial exon cell line
(Duda et al., 2014) 2014 K562, HCT116 ZFN(eGFP) KI efficiency (2A)
CRISPR(GFP)
(Mariano et al., 2014) 2014 HEK-293 TALEN(GFP, Cherry) Gene editing
(Geisinger et al., 2016) 2015 HEK293, C2C12 CRISPR KiBL, NHEJ
H9-, JF10 hESCs Clover, Cerulean, Cherry
(He et al., 2016) 2016 H1, LO2, HEK293 CRISPR (GfP) Generating reporter
HCT116, BeL-7404 cell line
(Panza et al., 2015) 2015 HeLa Chromobodies Endogenous protein dynamics
(Binan et al., 2016) 2015 ARPE-19 Single cell Phenotypic analysis
laser tag
(Miyanari et al., 2013) 2013 E14, SF1 TALEs Visualising endogenous
(fluorescent) genomic sequences
(Ma et al., 2015) 2015 U2OS CRISPR
(Nelles et al., 2016) 2015 D. rerio CRISPR Protein dynamics
(Nelles et al., 2016) 2016