Single Cell Dynamics

Reference list:

Reference Year Model Method Aim
(Lackner et al., 2015) 2015 HAP1 CRISPR/Cas9 (TurboGFP) Endogenous gene expression
NanoLuc Subcellular localisation
(Xi et al., 2015) 2015 HEK-293 CRISPR/Cas9 (eGFP-Puro) Fluorescent microscopy
N-terminal FLAG-SNAP tag (TERT)
(Tanenbaum et al., 2014) 2014 HEK-293 Antibody-based (GFP) Fluorescent microscopy
CRISPR-Cas9: synthetic transc. factor Signal amplification
(Leonetti et al., 2016) 2016 HEK-293T CRISPR/Cas9 (Split GFP system) Proteome-wide analysis
GFP11
(Deng et al., 2015) 2015 MEFs, HeLa Nuclease-deficient CRiSPR/Cas9 Labeling sequence-specific
as probes loci fluorescently
(Kwon et al., 2015) 2015 Porcine parthenogenetic CRISPR/Cas9 Oct4 GFP Protein dynamics
zygotes Knock-in
(Ratz et al., 2015) 2015 U2OS CRISPR/Cas9 GFP Super resolution
Knock-in microscopy
(Chen et al., 2013) 2013 HEK-293T CRISPR/Cas9 Imaging genomic loci
eGFP-tagged endonuclease- in living human cells
deficient Cas9
(Stewart-Ornstein et al., 2016) 2016 MCF-7 CRISPR/Cas9 Protein dynamics in
eFLuT tagging system single cells
(Böttcher et al., 2014) 2014 D. melanogaster CRISPR/Cas9 Chromosomal gene
S2 cells GFP modification
(Chen et al., 2016) 2015 HEK-293T, RPE CRISPR/Cas9 Tagging multiple loci
Telomere, MUC4E
(Issaeva et al., 2010) 2010 H1299 Directed Central Dogma Generating reporter
tagging - artificial exon cell line
(Duda et al., 2014) 2014 K562, HCT116 ZFN(eGFP) KI efficiency (2A)
CRISPR(GFP)
(Mariano et al., 2014) 2014 HEK-293 TALEN(GFP, Cherry) Gene editing
(Geisinger et al., 2016) 2015 HEK293, C2C12 CRISPR KiBL, NHEJ
H9-, JF10 hESCs Clover, Cerulean, Cherry
(He et al., 2016) 2016 H1, LO2, HEK293 CRISPR (GfP) Generating reporter
HCT116, BeL-7404 cell line
(Panza et al., 2015) 2015 HeLa Chromobodies Endogenous protein dynamics
(Binan et al., 2016) 2015 ARPE-19 Single cell Phenotypic analysis
laser tag
(Miyanari et al., 2013) 2013 E14, SF1 TALEs Visualising endogenous
(fluorescent) genomic sequences
(Ma et al., 2015) 2015 U2OS CRISPR
(Nelles et al., 2016) 2015 D. rerio CRISPR Protein dynamics
(Nelles et al., 2016) 2016 U2OS CRISPR RNA tracking in live cells
(Anton et al., 2014) 2014 mouse ESCs CRISPR Visualisation of DNA sequences

References

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  2. Linghe Xi, Jens C. Schmidt, Arthur J. Zaug, Dante R. Ascarrunz, Thomas R. Cech. A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biol 16 Springer Science \(\mathplus\) Business Media, 2015. Link

  3. ME Tanenbaum, LA Gilbert, LS Qi, JS Weissman, RD Vale. A protein-tagging system for signal amplification in gene expression and fluorescence imaging.. Cell 159, 635-46 (2014).

  4. MD Leonetti, S Sekine, D Kamiyama, JS Weissman, B Huang. A scalable strategy for high-throughput GFP tagging of endogenous human proteins.. Proc Natl Acad Sci U S A 113, E3501-8 (2016).

  5. W Deng, X Shi, R Tjian, T Lionnet, RH Singer. CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells.. Proc Natl Acad Sci U S A 112, 11870-5 (2015).

  6. J Kwon, S Namgoong, NH Kim. CRISPR/Cas9 as tool for functional study of genes involved in preimplantation embryo development.. PLoS One 10, e0120501 (2015).

  7. M Ratz, I Testa, SW Hell, S Jakobs. CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells.. Sci Rep 5, 9592 (2015).

  8. B Chen, LA Gilbert, BA Cimini, J Schnitzbauer, W Zhang, GW Li, J Park, EH Blackburn, JS Weissman, LS Qi, B Huang. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.. Cell 155, 1479-91 (2013).

  9. Jacob Stewart-Ornstein, Galit Lahav. Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Cell Reports 14, 1800–1811 Elsevier BV, 2016. Link

  10. R Böttcher, M Hollmann, K Merk, V Nitschko, C Obermaier, J Philippou-Massier, I Wieland, U Gaul, K Förstemann. Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.. Nucleic Acids Res 42, e89 (2014).

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  14. A Mariano, L Xu, R Han. Highly efficient genome editing via 2A-coupled co-expression of two TALEN monomers.. BMC Res Notes 7, 628 (2014).

  15. JM Geisinger, S Turan, S Hernandez, LP Spector, MP Calos. In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining.. Nucleic Acids Res 44, e76 (2016).

  16. X He, C Tan, F Wang, Y Wang, R Zhou, D Cui, W You, H Zhao, J Ren, B Feng. Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair.. Nucleic Acids Res 44, e85 (2016).

  17. P Panza, J Maier, C Schmees, U Rothbauer, C Söllner. Live imaging of endogenous protein dynamics in zebrafish using chromobodies.. Development 142, 1879-84 (2015).

  18. L Binan, J Mazzaferri, K Choquet, LE Lorenzo, YC Wang, el B Affar, Koninck Y De, J Ragoussis, CL Kleinman, S Costantino. Live single-cell laser tag.. Nat Commun 7, 11636 (2016).

  19. Y Miyanari, C Ziegler-Birling, ME Torres-Padilla. Live visualization of chromatin dynamics with fluorescent TALEs.. Nat Struct Mol Biol 20, 1321-4 (2013).

  20. H Ma, A Naseri, P Reyes-Gutierrez, SA Wolfe, S Zhang, T Pederson. Multicolor CRISPR labeling of chromosomal loci in human cells.. Proc Natl Acad Sci U S A 112, 3002-7 (2015).

  21. DA Nelles, MY Fang, MR O’Connell, JL Xu, SJ Markmiller, JA Doudna, GW Yeo. Programmable RNA Tracking in Live Cells with CRISPR/Cas9.. Cell 165