Design and construction of a semi-cycle system of oxygen supplied
intensification using hydrogen peroxide for high-performance glucose
oxidation
Abstract
In the past decade, glucose conversion has attracted increasing
attention for use in both biosensor preparation and gluconic acid
synthesis. However, differences in glucose concentration have led to
different research priorities. Here, we sought to study the limited
conditions for synthesizing high concentrations of gluconate using
glucose oxidase and catalase. Considering product inhibition and the
oxygen supplied limitations posed by gluconic acid and dissolved oxygen
(DO), we present here a method using continuous glucose flow to the
system along with the addition of H2O2 to the system as an oxygen
precursor. Then the two features were coupled, constructing a novel,
semi-cycle oxygen regeneration system to improve the product space-time
yield. The effects of glucose and H2O2 addition on reaction rate were
tested; the amount of gluconate yield was also assessed. Results
indicated that glucose conversion was consistent with the DO value of
the system; moreover, that the gluconate space-time yield was up to 61.3
g/L/h—36-fold increase when compared with unregulated gluconate
synthesis. Collectively, these findings identified reaction conditions
that use H2O2 as an oxygen precursor for suppling oxygen, thus guiding
the design of an enzymatic reaction that using oxygen for its enzyme
catalyzed system.