Purpose: Along with the increase in the frequency of multiple pregnancies, an increase is observed in the frequency of feto-maternal negativities. In our study, we aimed to determine the levels of iron, ferritin, hemoglobin, zinc, and copper in second-trimester multiparous twin pregnants and to compare them with values in women with the same demographic characteristics. Methods: Three groups were created in the study; control group, single pregnancy group, and twin pregnancy group. Venous blood samples were taken from individuals after 12 hours of fasting. Iron, zinc, copper, and ferritin levels were measured. Results: Compared with the control group, the Fe, Ferritin, and Zn values of the individuals in the single and twin pregnancy groups were statistically low while Cu levels were significantly high (p<0.05). Also, when compared with individuals in a single pregnancy group, a statistically significant decrease was found in Fe, Ferritin, and Zn levels in the twin pregnancy group (p<0.05); Although there was a minimal increase in Cu levels, this increase was not statistically significant. Conclusion: Since changes in trace element levels can lead to feto-maternal adverse effects, we think that dietary habits should be monitored, and zinc, copper, and iron levels should be followed in pregnant women.