In the current study, the waste-to-wealth concept has been applied. We were focused on using waste palm oil sludge to isolate and characterize various microorganisms that produce various industrial significant enzymes, such as polyphenol oxidases. We were able to isolate several bacteria that were discovered to be tyrosinase producers: Bacillus cereus, Acinobacter seifertii, Klebsiella variicola, and Pseudomonas stutzeri. Laccase producers Trametes polyzona, Bacillus subtilis, Bacillus pumilus, and Staphylococcus condimenti, as well as lipase producers. After they were grown for 18 hours at 35°C, pH 6.0, with substrates of 0.1% casein and 2.0% glucose, it was confirmed that they produce industrially important enzymes. Further, we focused specifically on B. cereus because it was evident that it produces tyrosinase. Lipase is another targeted enzyme, and S. condimenti was discovered to be a hyperproducer. The production conditions included are 24-hour incubation period at 40°C and pH 6.0, while typical substrates like starch and coconut oil were employed. 43 kDa-identified lipase that was found to be active at pH 7.0 and 40°C. Salts like NaCl, different detergents like Triton X-100, and Tween-80, and many metal ions all enhanced the activity, making the enzyme unique in its biological function. Only 40% inhibition has been seen, even with EDTA (2.5 mM), which does not completely block its function. Very few organic solvents such as butanol, acetone, and DMF are involved in inhibiting its activity.