Decoding the chromosome-scale genome of the nutrient-rich Agaricus
subrufescens: A Resource for fungal biology and biotechnology.
Abstract
Agaricus subrufescens, also known as the “mushroom of the sun,”
has significant nutritional and medicinal value. However, its short
shelf life due to the browning process results in post-harvest losses
unless it’s quickly dehydrated. This restricts its availability to
consumers in the form of capsules. A genome sequence of A.
subrufescens may lead to new cultivation alternatives or the
application of gene editing strategies to delay the browning process. We
assembled a chromosome-scale genome using a hybrid approach combining
Illumina and Nanopore sequencing. The genome was assembled into 13
chromosomes and 31 unplaced scaffolds, totalling 44.5 Mb with 96.5%
completeness and 47.24% GC content. 14,332 protein-coding genes were
identified, with 64.6% of the genome covered by genes and 23.41%
transposable elements. The mitogenome was circularized and encoded
fourteen typical mitochondrial genes. Four PPO genes and the Mating-type
locus were identified. Phylogenomic analysis supports the placement of
A. subrufescens in the Agaricomycetes clade. This is the first available
genome sequence of a strain of the “mushroom of the sun.” Results are
available through a Genome Browser
(https://plantgenomics.ncc.unesp.br/gen.php?id=Asub) and can support
further fungal biological and genomic studies.