Cold agglutinins are autoantibodies that can cause agglutination or clumping of red blood cells (RBCs) at temperatures below normal body temperature. This case report discusses a 37-year-old male patient who suffered from multiple injuries due to a motorcycle accident. The patient's laboratory tests revealed a high level of cold agglutinins, which resulted in abnormal RBC parameters. The study aims to investigate the impact of cold agglutinins on RBC parameters in a trauma patient. The findings of this case report highlight the importance of recognizing cold agglutinins in trauma patients to avoid misinterpretation of laboratory results.