Ultra-high pressure (UHP) metamorphism of the Tso Morari coesite-eclogite during burial in NW Himalaya has been intensively studied over the past several decades. However, amphibolite-facies metamorphism and accompanying metasomatism occurring at lower-crustal depths in the Tso Morari terrane are less well-constrained. In this study, we characterize the eclogite amphibolization and related metasomatic fluids by systematically sampling and analyzing the eclogites at the core of an eclogite boudin and the amphiblolized eclogite (amphibolite) at the rim. Integrated techniques including modal mineralogy, mineral chemistry, whole-rock geochemistry, Mössbauer spectroscopy, and thermodynamic modelling are used to constrain the fluid-induced eclogite amphibolization and associated fluid behaviors. Petrographic observations show that infiltration of an external fluid caused complete amphibolite-facies overprinting of the eclogites at the boudin rim. This is recorded petrographically as increased modal proportions of amphibole, biotite, epidote, plagioclase, and calcite in the amphibolites. The infiltrating fluid caused increased K₂O and CO₂ concentrations and higher bulk-rock Fe³⁺/ΣFe ratio for the amphibolites, as well as increased LILE (e.g., K, Rb, Cs, Sr, Ba) and ratios of Ba/Rb and Cs/Rb. Phase equilibria modelling using P–T–M(H₂O) pseudosections on the amphibolite and the surrounding gneiss indicate that the fluid infiltration occurred at 9.0–12.5 kbar and ~608 °C with >2.6–3.1 mol % H₂O infiltration. The abrupt increase of bulk-rock Fe³⁺/ΣFe ratio from 0.192 to 0.395 near the boudin rim indicate that this phase of fluid most likely derived from the mixing of dehydrated host orthogneiss and/or metasediments during uplift at the amphibolite-facies zone in the subduction channel. This study also demonstrates the need for using careful petrographic observations and geochemical analysis in parallel with thermodynamic modelling to achieve realistic results.