High-quality haplotype-resolved genome assemblies of ring-cup oak
(Quercus glauca) provide insight into the demographic dynamics of a
dominant tree in East Asia subtropics evergreen broadleaved forests
- Chang-Sha LUO,
- Tian-Tian Li,
- Ying Song,
- Ting-Ting Fan,
- Xiang-Bao Shen,
- Rong Yi,
- Xiao-Ping Ao,
- Gang-Biao XU,
- Xiao-Long JIANG,
- Min DENG
Tian-Tian Li
Central South University of Forestry and Technology
Author ProfileYing Song
Central South University of Forestry and Technology
Author ProfileTing-Ting Fan
Central South University of Forestry and Technology
Author ProfileXiang-Bao Shen
Central South University of Forestry and Technology
Author ProfileRong Yi
Central South University of Forestry and Technology
Author ProfileXiao-Ping Ao
Central South University of Forestry and Technology
Author ProfileGang-Biao XU
Central South University of Forestry and Technology
Author ProfileXiao-Long JIANG
Central South University of Forestry and Technology
Author ProfileAbstract
Quercus section Cyclobalanopsis is a dominant woody lineage in East
Asian evergreen broadleaved forests. Regardless of its significant
ecological and economic importance, little was known on the genome of
this unique oak group. Quercus glauca, also known as ring-cup oak, is
the most widespread tree in section Cyclobalanopsis. In this study, a
high-quality haplotype-resolved reference genome of Q. glauca was
generated from PacBio CCS and Hi-C reads. The genome size, contig N50,
and scaffold N50 of Q. glauca are 902.8 Mb, 7.6 Mb, and 69.3 Mb,
respectively, for haplotype1, and 913.2 Mb, 7.2 Mb, and 71.5 Mb,
respectively, for haplotype2. The LTR Assembly Index of the Q. glauca
genome was more than 22. A total of 37,460 and 38,312 protein-coding
genes were predicted in haplotype1 and haplotype2, respectively.
Homologous chromosomes of Q. glauca had excellent gene pair
collinearity. A total of 905 R-genes were detected in Q. glauca. The
divergence between Q. glauca and Q. suber occurred about 44.6 million
years ago, agreeable to the earliest fossil records of section
Cyclobalanopsis found in East Asia. The global climate change before the
late Miocene and local climate change since the Pliocene were the main
causes of the effective population size change of Q. glauca. The
high-quality genome assembly of the most widespread species of section
Cyclobalanopsis can provide essential genomic resources for unraveling
the evolution mystery of the main oak lineages, and to promote the
application of genomic data on the interspecific introgression, local
adaptation, and speciation studies of oaks.