Domen Pregeljc1, Janja Skok1, Tina Vodopivec1, Nina Mencin1, Andreja Krušič1, Jure Ličen1, Kristina Šprinzar Nemec1, Aleš Štrancar1, Rok Sekirnik1,*1BIA Separations d.o.o., a Sartorius company, Mirce 21, 5270 Ajdovščina, Slovenia*correspondence: Rok Sekirnik, BIA Separations d.o.o., Mirce 21, 5270 Ajdovščina, SloveniaTel.: +386 59 699 500; Fax: +386 59 699 599E-mail: [email protected]: mRNA; in vitro transcription; HPLC; chromatography; analytics; vaccines.Abbreviations:ARCA: Anti-reverse cap analogDTT: dithiothreitolEDTA: Ethylenediaminetetraacetic acidGFP: green fluorescent proteinGTP: guanidine triphosphateHPLC: high pressure liquid chromatographyIVT: in vitro transcriptionLNP: lipid nanoparticleNTP: Nucleoside TriphosphatemRNA: messenger ribonucleic acidRNAP: RNA polymerase enzyme