Objective: Vitamin D deficiency is claimed to be associated with many diseases in the world populations. Many studies reported that vitamin D deficiency is quite prevalent in Pakistan. The aim of this review is to describe the current status of vitamin D deficiency in healthy individuals and in different disease conditions in this country. Methods: Search engines such as “Google Scholar”, “Medline and PubMed” were used by applying key words such as vitamin D deficiency, vitamin D deficiency and different disease conditions in Pakistani population. Results: Total 82 studies were included in which 12 studies showed vitamin levels less than 30 ng/mL in the healthy individuals (male and female) and 70 studies in different disease conditions. It found that about 64.6% healthy subjects had vitamin D levels less than 30 ng/mL. Moreover, 70 studies showed its deficiency in some diseases such as musculoskeletal, bone and periodontal problems, nursing mothers and children, tuberculosis, diabetes, cardiovascular problems and some type of cancers. Conclusion: The current status of vitamin D deficiency in healthy population and in several diseases and conditions are thoroughly discussed in this review. This review could be helpful to understand status of vitamin D and different aspects of vitamin D research in this population