Background: This study aimed to investigate the Hospital-Acquired Conditions (HACs) and find deficits in our hospital, so as to improve the incidence of HACs in China from our retrospect. Methods: We conducted statistics on the incidence and the percentage of HACs, and managed the cost and length of stay of the 950 patients went through HACs in our hospital from 2016 to 2018. Results: The overall incidence was 0.571%. The two most common conditions with the most cost were deep vein thrombosis (DVT) and pulmonary embolisms (PE) (351), and surgical site infections (SSI) (143), which were followed closely by manifestations of poor glycemic control (53), vascular catheter–associated infections (VCAIs) (52), stage III and IV pressure ulcers (49), catheter-associated urinary tract infections (CAUTIs) (33), and falls and trauma (30). Iatrogenic pneumothorax with venous catheterization (7) only occupied small portions. Blood incompatibility and air embolism didn’t happen in the three years. Conclusions: From the comparison of three years, the condition decreased in 2017, while met a higher increase in 2018. This may relative to the launch of medical reform in 2017. Chinese hospitals still need to investigate the HACs, give adaptable emphasis and concern to improve, and promote the overall level of medical management.