Cytochrome Oxidase I based haplotype diversity of Mahseer (Tor tor) from
eight sites in Southern Rajasthan
Abstract
Mahseer (Tor tor) is an important game fish in southern Rajasthan,
India, and has recently been listed as an endangered species. The
present study aimed to provide robust taxonomic classification of the
species by calculating the haplotype gene frequency of Mahseer
indigenous to the water bodies of southern Rajasthan. Thirty-two Tor tor
samples were collected from eight sites in southern Rajasthan, India.
The Cytochrome oxidase I (COI) gene was sequenced from all 32 samples.
Haplotype frequencies of the samples collected were measured and
phylogenetic analysis was performed. We observed that the Tor tor
samples collected from the eight sites could be divided into three
haplotypes, hap_1, hap_2, and hap_3. Samples collected from five
sites (Chambal river, Rana Pratap Sagar, Bassi dam, Kali Sindh river,
and Parwan river) exhibited the same COI haplotype, hap_1. Tor tor
hap_2 was found only in samples from Badi lake; samples from Daya dam
and Madar tank exhibited only Hap_3, giving a clear idea about
phylogeny and group affiliations. This data can be used while
repopulating the fish, which will contribute to better ecosystem
management.