Hemicellulose-rich substrates produced in the lignocellulose biorefinery context can yield macromolecular hemicellulose structures with assorted application in the chemical industry. In the current work, alkaline-active xylanase aided to extract a varied group of xylan fractions from alkaline-sulfite pretreated sugarcane bagasse. Extraction yields (12-44%) depended on the reaction time and the xylanase load used in the extraction procedure. Prepared fractions contained low levels of lignin contamination (4-9%) and 4-O-methyl glucuronic acid arabinoxylan structures. Molar masses of fractions ranged from 2.3 kDa to 34 kDa. Prepared xylans were incorporated onto eucalyptus pulp fibers up to 4.7 g xylan/100 g pulp. The efficiency of xylan incorporation was dependent on the xylan structures, where low molar mass and low substitution degree favored high incorporation levels. Enzymatic xylan extraction proved a distinguishing procedure to add value in the lignocellulosic biorefinery chain of sugarcane bagasse once extracted xylans are suitable for incorporation onto cellulosic fibers.