Efficient production of isomalto-oligosaccharides by cell associated
transglucosidase of isolated strain Debaryomyces hansenii using
microfiltration membrane
Abstract
An efficient recycling method of microfiltration membrane was used to
develop the continuous production of isomalto-oligosacharides (IMOs) by
cell associated transglucosidase(TG) of a novel strain, D.hansenii from
maltose. Transglucosidase synthesized seventy types of microbial strains
were screened,isolated and investigated the efficiency of IMOs
production. The most potent transglucosidase producer was identified as
Debaryomyces hansenii using 18s rRNA sequencing. Media optimization
preliminary studies were performed to maximize the synthesize of
transglucosidase and biomass yield (54 g L-1). Parameters optimization
studies were investigated using whole cells of D.hansenii
(~4023 units L-1 α-glucosidase activity) from 10 L
fermenter to increase the transglucosidase activity through
biotransformation. IMOs was continuously synthesised by reusing the cell
biomass (6%, w/v) in a 3L bioreactor using microfiltration membrane
system with 30% (w/v) maltose concentration under controlled
temperature of 34°C in9 to 18h for 5 cycles. The isolated yeast strain,
D.hansenii was found to utilize more than 98% maltose with higher
conversion efficiency for production of IMOs with 65% purity, 85%
yield and productivity of 48.8 g L-1.h which confirmed by HPLC.