Pyridoxine (vitamin B6) is widely used as a dietary supplement for the prevention and treatment of vitamin B6 deficiency, anemia, cardiovascular disease, tuberculosis, etc. The objective of this research study was to evaluate the impact of the Trivedi Effect® on the physicochemical properties of vitamin B6 using sophisticated analytical techniques. Vitamin B6 hydrochloride was divided into two parts, i.e., control and treated parts. Only the treated part has received the Consciousness Energy Healing Treatment (Biofield Energy Healing Treatment) remotely by a well-known Biofield Energy Healer, Dahryn Trivedi. The powder XRD relative peak intensities and crystallite size of the treated vitamin B6 were significantly altered ranging from -14.27% to 31.89% and -16.68% to 49.87%, respectively compared to the control sample. The average crystallite size of the treated vitamin B6 was significantly increased by 5.66% compared with the control sample. The particle size values of the treated vitamin B6 were significantly altered by -11.90% (d10), 5.92% (d50), 20.42% (d90), and 19.05% {D(4,3)}; whereas, the specific surface area of the treated vitamin B6 was significantly increased by 5.49% compared with the control sample. Similarly, the latent heat of fusion of the treated vitamin B6 was significantly increased by 34.29% compared with the control sample. The total weight loss and the maximum thermal degradation temperature in the treated vitamin B6 were slightly increased compared with the control sample. The Trivedi Effect® might have produced a new polymorphic form of vitamin B6 which would be more soluble, more bioavailable and thermally more stable compared with the control sample. Thus, The Biofield Energy Treated vitamin B6 could be a more efficacious nutraceutical and/or pharmaceutical formulations against vitamin B6 deficiency, cardiovascular disease, sideroblastic anemia, pulmonary tuberculosis, diabetes, Alzheimer’s disease, pyridoxinedependency seizures, hyperhomocysteinemia, cancer, asthma, dysmenorrhea, etc.