L-tryptophan is an amino acid which helps in the formation of proteins and certain brain-signaling chemicals, etc. This study was carried out to investigate the impact of the Trivedi Effect® on the physicochemical properties of L-tryptophan by using modern analytical techniques. For analysis, the sample was divided into control and treated parts. In which control part didn’t received any Biofield Energy Treatment, while the treated part was received the Trivedi Effect®-Consciousness Energy Healing Treatment was remotely by a famous Biofield Energy Healer, Alice Branton. The values of particle size in the treated sample was increased by 1.52% (d10), 1.24% (d50), 4.33% (d90), and 3.31% {D(4,3)}, while the specific surface area was reduced by 1.05% as compared to the control sample. The treated L-tryptophan shown that the powder XRD peak intensities and crystallite sizes were altered, ranging from -87.32% to 20.88% and -9.54% to 614.29%, respectively, compared to the control sample. Moreover, the average crystallite size of the treated L-tryptophan was increased by 25.97% than the control sample. The overall weight loss of the treated L-tryptophan was reduced by 5.27%, in comparison to the control sample. It resulted in a significant 228.88% increase in the residual amount of the treated L-tryptophan, as compared to the untreated sample. Besides, the DTG thermograms of both the samples showed two peaks and the maximum thermal degradation temperature corresponding to 1st and 2nd peak of the treated sample was significantly altered by 25.34% and -1.45%, respectively as compared to the control sample. The melting temperature of the treated sample was increased by 2.69%, while the ΔHfusion was reduced by 16.36%, as compared with the control sample. The Consciousness Energy Healing Treatment might act in creating some novel polymorphic form of L-tryptophan that might prove to be beneficial in terms of improved flowability, appearance, and thermal stability as compared to the untreated sample. Thus, the use of the Biofield Energy Treated L-tryptophan might be considered as an advantageous approach for designing the more efficacious nutraceutical/pharmaceutical formulations.