Systemic inflammatory response syndrome (SIRS) is a multiplex pathophysiological defense response against noxious stressor such as infection, trauma, burns, or any others injuries. Study objective was to evaluate the antioxidants and anti-inflammatory potential of Biofield Energy Treated (Blessed) Proprietary Test Formulation and Biofield Energy Healing Treatment (Blessing) per se to the animals on Cecal Slurry, LPS, and E. coli-induced SIRS model in Sprague Dawley rats. Each component of the test formulation was divided into two parts; one part was denoted as untreated test formulation, while other part of the test formulation and three group of animals received Biofield Energy Healing Treatment remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of MPO was significantly (p≤0.001) reduced by 51.44%, 71.69%, 55.79%, 55.16%, and 58.12% in G5 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation); G6 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se to animals from day -15); G7 (Cecal Slurry, LPS and E. coli + Biofield Energy Treated test formulation from day -15); G8 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se + Biofield Energy Treated/Blessed test formulation from day -15), and G9 (Cecal Slurry, LPS and E. coli + Biofield Energy Treatment per se animals + untreated test formulation) groups, respectively with reference to disease control (G2) group. The level of LPO end product in terms of malondialdehyde (MDA) was significantly (p≤0.001) reduced by 52.71%, 56.54%, 67.35%, and 75.28% in G6, G7, G8, and G9 as compared to the G4 group. The level of MMP-9 was significantly (p≤0.001) decreased by 34.79%, 48.57%, 39.29%, and 41.25% in G6, G7, G8, and G9, respectively with reference to G4 group. Moreover, the level of FDP was significantly (p≤0.001) decreased by 39.87%, 44.91%, 39.76%, 43.09%, and 46.47% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. The level of substance P was significantly (p≤0.001) decreased by 19.93%, 25.51%, and 27.92% in the G7, G8, and G9 groups, respectively as compared to the G4 group. The level of iNOS was significantly (p≤0.001) decreased by 39.26% (p≤0.001), 38.95% (p≤0.001), 47.63% (p≤0.001), and 59.78% (p≤0.001) in the G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested the antioxidant and anti-inflammatory potentials of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se with respect to various inflammatory conditions that might be beneficial various types of systemic inflammatory disorders specially sepsis, trauma, septic shock or any types of injuries. Consequently, the results significantly slowdown the inflammation-related symptoms in preventive treatment groups like G6, G7, G8, and G9.