The present study was aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/Item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on cytokine (IL-6 and TNF-α) levels in Human monocytic cell line (THP-1) cell line in RPMI. Each constituents of test formulation were divided into two parts; one section was defined as the untreated test formulation (UT), while the other portion of the test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing Treatment/Blessing and divided as Biofield Energy Treated (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range 77% to 134%. IL-6 level was significantly inhibited by 48.7% and 21.1% at 5 and 10μg/mL, respectively in the BT-RPMI+UT-TI group, while 11.8%, 20.8%, and 44% inhibition of IL-6 was reported at 0.01, 5, and 10μg/mL respectively, in the UT-RPMI+BT-TI group, and 422.9%, 84.2%, and 14.7% inhibition of IL-6 at 0.01, 0.1, and 10μg/mL, respectively in the BT-RPMI+BT-TI group as compared with the untreated test formulation and RPMI group. Similarly, TNF-α inhibition by 26.1%, 29.2%, and 155.2% in the UT-RPMI+BT-TI group at 0.01, 0.1, and 5μg/mL respectively, as compared with the untreated group. However, BT-RPMI+UT-TI group showed a significant inhibition of TNF-α by 50% and 14.9% at 0.01 and 10μg/mL, respectively, while BT-RPMI+BT-TI group showed a significant inhibition of TNF-α by 46.4%, 17.5%, 21.2%, and 228.4% at 0.01, 0.1, 1, and 10μg/mL, respectively as compared with the untreated test formulation and RPMI group. Overall, the in vitro cell line data suggested significance inhibition of pro-inflammatory cytokines (TNF-α and IL-6) at all the testes concentration of Biofield Energy Treated test formulation. Overall, the experimental results showed the significant improved immune profile by regulation of cytokines profile, which can be used against immunity disorders, mental illness, stress, lethargic conditions, alertness, migraine, minimizing toxic effects of free radicals, and many other immune related disorders.