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Outcomes based on histological tumor necrosis and predictive clinical and laboratory parameters for necrosis in children with osteosarcoma treated on a non-High Dose Methotrexate based chemotherapy backbone
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  • BADIRA CHERIYALINKAL PARAMBIL,
  • Poonam Khemani,
  • Ajay Puri,
  • Ashish Gulia,
  • Maya Prasad,
  • Venkata Gollamudi,
  • Mukta Ramadwar,
  • Bharat Rekhi,
  • Poonam Panjwani,
  • Prakash Nayak,
  • Manish Pruthi,
  • Nilendu Purandare,
  • Amit Janu,
  • Akash Pawar,
  • Komal Adhav,
  • Girish Chinnaswamy,
  • Sajid Qureshi
BADIRA CHERIYALINKAL PARAMBIL
Tata Memorial Centre
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Poonam Khemani
Tata Memorial Centre
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Ajay Puri
Tata Memorial Centre
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Ashish Gulia
Tata Memorial Centre
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Maya Prasad
Tata Memorial Centre
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Venkata Gollamudi
Tata Memorial Centre
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Mukta Ramadwar
Tata Memorial Centre
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Bharat Rekhi
Tata Memorial Centre
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Poonam Panjwani
Tata Memorial Centre
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Prakash Nayak
Tata Memorial Centre
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Manish Pruthi
Tata Memorial Centre
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Nilendu Purandare
Tata Memorial Centre
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Amit Janu
Tata Memorial Centre
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Akash Pawar
Tata Memorial Centre
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Komal Adhav
Tata Memorial Centre
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Girish Chinnaswamy
Tata Memorial Centre

Corresponding Author:[email protected]

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Sajid Qureshi
Tata Memorial Centre
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Abstract

1 Background Histopathological response to neoadjuvant-chemotherapy(NACT) measured as tumor necrosis(TN) has been reported to be prognostic of outcomes post HDMTX- based chemotherapy. We studied outcomes based on different cut-offs of TN and delineated clinical-laboratory parameters predictive of TN on a non-HDMTX chemotherapy backbone. 2 Materials and Methods Children ≤15years, with osteosarcoma treated on OGS-2012 protocol and surgery post-NACT from January 2013-December 2020 were retrospectively analysed. TN was reported as percentage necrosis. Kaplan-Meier, log-rank, Pearson’s Chi-square tests were used. 3 Results Analysis was done in 258 patients. Median age-12years(range,3-15years), M:F-1.7:1. Amputation was performed in 20.1%. Median TN was 94%. At a median follow-up of 38months(range,34-45months), 3year Event Free Survival(EFS) and Overall Survival(OS) of the whole cohort were 56.1%(SE,3.3%) and 87.8%(SE,2.4%). For entire cohort, TN-70%(29.3%vs60.7%), 90% (38.7%vs69.0%), 100%(50.8%vs84.1%), were prognostic for EFS(p=0.0001), while TN-90%(80.3%vs92.9%,p=0.006) and 100%(85.5%vs97.7%,p=0.023) were prognostic for OS. For localized disease, TN-70%(35.4%vs 66.4%), 90%(41.6%vs77.0%), 100%(54.8%vs96.2%) were prognostic for EFS(p=0.0001), and OS(p=0.0001). For metastatic disease, TN-70% was prognostic for EFS(16.6%vs50.1%,p=0.0047). Receptor-Operator Curve derived cut-off of 85.5%TN for EFS, 83.5%TN for OS prognosticated whole and localized cohorts the best. For metastatic cohort, 84.5%TN best prognosticated EFS. Among clinical-laboratory parameters, male gender(OR:1.9,p=0.01), amputation (OR:2.1,p=0.014) had a higher risk of <90%TN. 4 Conclusions Tumor necrosis at 90% cut-off in localized disease is prognostic of survival on a non-HDMTX based backbone, though best outcomes are seen with 100%TN, but 70%TN and other cut-offs require further exploration. A lower cut-off of 70%(or other) in metastatic disease could be used for prognostication. Amputation, male gender predicts poor histological necrosis.