The Hoechst Side Population Cells can be Effectively Detected by 375nm
and 405nm Lasers
Abstract
The side population (SP) cells are identified through Hoechst 33342
staining and analyzed using flow cytometry (FCM). The Hoechst SP method
is utilized for the isolation of stem cells based on the dye efflux
properties of ATP-binding cassette (ABC) transporters. This method was
initially employed for the identification and isolation of hematopoietic
stem cells (HSCs), but it has now evolved to primarily focus on the
identification and isolation of cancer stem cells (CSCs). In this study,
the capability of the 375 nm and 405 nm lasers in detecting SP cells was
assessed and compared with that of the 355nm laser. Our findings
demonstrate a remarkable similarity between the SP cells detected by the
355nm laser and the 405nm laser on the CytoFLEX system of FCM. These
results suggest that the high-power 375 nm and 405 nm lasers can serve
as a viable alternative to the 355 nm laser for SP cell detection.