loading page

Development of disposable electrode for the detection of mosquito-borne viruses
  • +2
  • Fahmida Nasrin,
  • Indra Mwmdi Khoris,
  • Ankan Dutta Chowdhury,
  • Sjakurrizal El Muttaqein,
  • Enoch Y. Park
Fahmida Nasrin
Shizuoka University
Author Profile
Indra Mwmdi Khoris
Shizuoka University
Author Profile
Ankan Dutta Chowdhury
Amity University - Kolkata Campus
Author Profile
Sjakurrizal El Muttaqein
Shizuoka University
Author Profile
Enoch Y. Park
Shizuoka University

Corresponding Author:[email protected]

Author Profile


Development of disposable, rapid and convenient biosensor with high sensitivity and reliability is the most desired method of viral disease prevention. To achieve this goal, in this work, a practical impedimetric biosensor has been implemented into a disposable electrode on a screen-printed carbon electrode (SPCE) for the detection of two mosquito-borne viruses. The biosensor fabrication has step-wisely carried out on the disposable electrode surface at room temperature: starting from conductive film formation, physical binding of the gold nanoparticles (AuNPs)-polyaniline (PAni) into the conductive film, and biofunctionalization. To get the maximum efficiency of the antibody, biotinylated antibody has been conjugated on the surface of AuNP-PAni /PAni-SPCE via the streptavidin-biotin conjugation method which is a critical factor for the high sensitivity. Using the antibody-antigen interaction, this disposable electrode has designed to detect mosquito-borne infectious viruses, Chikungunya virus (CHIKV) and Zika Virus (ZIKV) separately in a wide linear range of 100 fg/mL to 1 ng/mL with a low detection limit of 1.33 fg/mL and 12.31 fg/mL, respectively.
16 Mar 2023Submitted to Biotechnology Journal
17 Mar 2023Submission Checks Completed
17 Mar 2023Assigned to Editor
17 Mar 2023Reviewer(s) Assigned
19 Mar 2023Review(s) Completed, Editorial Evaluation Pending
28 Mar 2023Editorial Decision: Revise Major
25 Apr 20231st Revision Received
25 Apr 2023Submission Checks Completed
25 Apr 2023Assigned to Editor
25 Apr 2023Reviewer(s) Assigned
26 Apr 2023Review(s) Completed, Editorial Evaluation Pending
27 Apr 2023Editorial Decision: Accept