The differences in the functional diversity and species diversity of macroinvertebrates can be based to evaluate the changes in local environment. However, there are little available analysis on the effect mechanism of seasons on the functional characteristics of macroinvertebrate communities in the subtropical region. This work compared the functional feeding groups (FFG) of macroinvertebrates in wet season, normal season, and dry season of 2021 in Jingui River in Shenzhen. This work mainly was aimed to comprehend the connection between the environmental driving elements in the Jingui River and the seasonal distribution of the FFG of macroinvertebrates. The highest species diversity and abundance were found among the collector-gatherers (GC), while the largest biomass was observed among the predators. Overall, the functional diversity of the Jingui River exhibited a significantly seasonal change. In particular, the functional diversity decreased in wet season, implying a stronger disturbance. A multiple regression analysis revealed that the species diversity and stream environmental parameters might account for 12.8% - 72.9% of the functional diversity of macroinvertebrates.