This study aimed to explore the effect of MEG3 genetic polymorphisms on telomere length (TL) in Polycyclic aromatic hydrocarbons (PAHs) exposure populations. The 544 PAHs-exposure workers and 238 controls were recruited, and urinary OH-PAHs concentrations were determined with high-performance liquid chromatography. The TL was measured using a quantitative polymerase chain reaction. The MEG3 genetic polymorphisms were detected by the flight mass spectrometry platform. The TL in the exposure group (4.57±0.84) was significantly lower than the controls (5.00±0.75), and TL had a negative correlation with OH-PAHs. Generalize linear model found that PAHs-exposure [β(95%CI) = -0.409(-0.537, -0.282), P <0.001], age (years) [β(95%CI) = -0.010(-0.019, -0.002), P = 0.018], and genotype CT+TT for MEG3 rs10132552 [β(95%CI) = -0.299(-0.582, -0.017), P = 0.038] were associated with the decreased TL. In conclusion, PAHs-exposure and genotype CT+TT for MEG3 rs10132552 may be the risk factors for TL. And TL may decrease with age, BMI, and urinary OH-PAHs.