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Assessments of TP53 and CTNNB1 gene hotspot mutations in circulating tumour DNA of hepatitis B virus-induced hepatocellular carcinoma
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  • Baibaswata Nayak,
  • Sonu Kumar,
  • Neeti Nadda,
  • Afnan Quadri,
  • Rahul Kumar,
  • Shashi Paul,
  • Pranay Tanwar,
  • Shivanand Gammanagatti,
  • Nihar Ranjan Dash,
  • Anoop Saraya,
  • * Shalimar
Baibaswata Nayak
All India Institute of Medical Sciences

Corresponding Author:[email protected]

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Sonu Kumar
All India Institute of Medical Sciences
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Neeti Nadda
All India Institute of Medical Sciences
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Afnan Quadri
All India Institute of Medical Sciences
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Rahul Kumar
All India Institute of Medical Sciences
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Shashi Paul
All India Institute of Medical Sciences
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Pranay Tanwar
All India Institute of Medical Sciences
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Shivanand Gammanagatti
All India Institute of Medical Sciences
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Nihar Ranjan Dash
All India Institute of Medical Sciences
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Anoop Saraya
All India Institute of Medical Sciences
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* Shalimar
All India Institute of Medical Sciences
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Abstract

Background: Hepatitis B virus (HBV) infection is one of the major causes of chronic liver disease which progresses from hepatitis to liver fibrosis, cirrhosis and hepatocellular carcinoma (HCC). Early detection and laboratory based screening test of HCC is still a major challenge. HBV induces hepatocarcinogenesis through viral genome integration, chromosomal aberrations and modulation of host signaling pathways. Molecular alterations of cancer hallmark genes occur during the process of hepatocarcinogenesis. These signatures may release into the circulation through . circulating tumor DNA (ctDNA). Detection of these mutations in ctDNA may serve as liquid biopsy marker for screening, early detection and prognosis of HCC for which this study was undertaken. Methods: Consecutive patients of CHB-HCC (n=80), chronic hepatitis B (n=35) and healthy (n=15) controls were included for blood sample collection. The ctDNA was isolated from serum. Amplification and sequencing TP53 exon 7 and β-catenin exon 3 was carried out for predominant mutations in the ctDNA of HCC patients. Highly sensitive dual-probe based droplet digital PCR (ddPCR) assays were performed for TP53 (p.R249M & p.R249S) and β-catenin (p.S45P) driver mutations in healthy, CHB-noncirrhotic, cirrhotic and HCC patients. Results: Both TP53 gene exon 7 and CTNNB1 gene exon 3 region was amplified and sequenced in 32 HCC patient whereas sensitive ddPCR assay TP53 (p.R249M & p.R249S) and β-catenin (p.S45P) mutation for all 130 subjects. In sanger sequencing TP53 c.746 G˃T, p.R249M mutation was predominant. In ddPCR assay, 58.75% of HCC patients (n=47) ctDNA had at least one driver mutation in the ctDNA. Combined TP53 and CTNNB1 mutation was observed in 12.5% of HCC patients. Increased mutation frequency was observed in CHB-cirrhotic. CHB-HCC than CHB-noncirrhotic and healthy subjects. Percentage mutant fraction was highest in CHB-HCC than only CHB patients. Significant association TP53. R249M with smoking was observed in CHB-HCC patients. Poor survival was observed in HCC patients with combined TP53 and CTNNB1 gene mutation. Conclusion: Driver mutation screening for TP53 and CTNNB1 gene can be done in ctDNA for early diagnosis and prognosis.