In Vitro proliferation of Petunia hybrida Vilm; Worldwide Important
Ornamental Plant
Abstract
This project tries to artificially grow one of the most ornamentally
significant Petunia hybrida when it is out of season. Murashige and
Skoog Basal Medium (MS Basal Medium) was used for in vitro growth of P.
hybrida, supplemented with different Plant Growth Regulators (PGRs).
Various physical and chemical factors on in vitro growth of P. hybrida
using different explants were analyzed. The type of auxin that proved
significant for somatic embryogenesis of P. hybrida Vilm was
2,4-Dichlorophenoxyacetic acid (2.0 mg/L) using the leaf as explant
under controlled environmental conditions, i.e., 92% at 23oC with a 5.8
pH and 16 hours photoperiod & 30g/l sucrose in. Friable calli were also
developed from leaf, node, and internodal explants on a tissue culture
medium (MS basal medium) when supplemented with other PGRs in certain
combinations i.e. 2,4-Dichlorophenoxyacetic acid (2.0 mg/L), Benzyl
Amino Purine (2.5mg/L), Benzyl Amino Purine + 2,4-Dichlorophenoxyacetic
acid (2mg/L +2.5mg/L), Naphthalene Acetic Acid (1.5mg/L),
2,4-Dichlorophenoxyacetic acid + Naphthalene Acetic Acid (2mg/L+1.5mg/L)
and Benzyl Amino Purine + Naphthalene Acetic Acid (2.5mg/L+0.5mg/L) at
optimal physical factors under strict aseptic environment. These results
may enable one to produce the required type of P. hybrida Vilm during
off and on seasons on a commercial scale by using plant tissue culture
as a tool.