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Unveiling the biological interface of protein complexes by mass spectrometry-coupled methods
  • Goeun Shin,
  • Sung In Lim
Goeun Shin
Pukyong National University

Corresponding Author:goeunshin625@gmail.com

Author Profile
Sung In Lim
Pukyong National University
Author Profile


Most biomolecules become functional and bioactive by forming protein complexes through interaction with ligands that are diverse in size, shape, and physicochemical properties. In the complex biological milieu, the interaction is ligand-specific, driven by molecular sensing and recognition of a binding interface localized within a protein structure. Mapping interfaces of protein complexes is a highly sought area of research as it delivers fundamental insights into proteomes and pathology and hence strategies for therapeutics. While X-ray crystallography and electron microscopy still serve as a gold standard for structural elucidation of protein complexes, artificial and static analytic nature thereof often results in a non-native interface that otherwise might be negligible or non-existent in biological environment. In recent years, the mass spectrometry-coupled approaches, chemical crosslinking (CLMS) and hydrogen-deuterium exchange (HDMS), have become valuable analytic complements to traditional techniques. These methods explicitly identify hot residues and motifs embedded in binding interfaces, in particular, for which the interaction is predominantly dynamic, transient, and/or caused by an intrinsically disordered domain. Here we review the principal role of CLMS and HDMS in protein structural biology with a particular emphasis on the contribution of recent examples to exploring biological interfaces. In addition, we describe recent studies that utilized these methods to expand our understanding of protein complex formation and related biological processes and to increase probability of structure-based drug design.
18 Jul 2022Submitted to PROTEINS: Structure, Function, and Bioinformatics
18 Jul 2022Submission Checks Completed
18 Jul 2022Assigned to Editor
18 Jul 2022Reviewer(s) Assigned
19 Oct 2022Review(s) Completed, Editorial Evaluation Pending
20 Oct 2022Editorial Decision: Revise Minor
28 Nov 20221st Revision Received
29 Nov 2022Submission Checks Completed
29 Nov 2022Assigned to Editor
29 Nov 2022Review(s) Completed, Editorial Evaluation Pending
12 Dec 2022Reviewer(s) Assigned
22 Dec 2022Editorial Decision: Accept
27 Dec 2022Published in Proteins: Structure, Function, and Bioinformatics. 10.1002/prot.26459