Seneca Valley Virus (SVV) infection was recently spread the pig farm in the Canada, American and China, human, mice and housefly have been the host and reservoirs. Although such cross-species transmission events result in limited onward in the new cross-species transmission, sustained outbreak have posed a new mammalian host. Thus, to determine whether mink was one of a new mammalian host in our study with the molecular characteristics of isolated SVA genomes, challenge, pathological study, and immune respond. Here, our research was the first systemic analysis on a new isolation strain of SVV from pig, the new strain infected the mink in oral and intestine, which produced the pathological change in the intestine. And the SVV could stimulate the specific neutralizing antibody. This study highlights the importance of identifying SVV infection in the mink and host as a mutational pressure for the virus evolution that could threaten livestock, public health and economic growth.