loading page

Sex Results in Divergence in Gut Bacterial Community between Female and Male Pardosa astrigera (Araneae: Lycosidae)
  • +2
  • Ying Gao,
  • Pengfeng Wu,
  • Shuyan Cui,
  • Abid Ali,
  • Guo Zheng
Ying Gao
Shenyang Normal University

Corresponding Author:gy434585296@163.com

Author Profile
Pengfeng Wu
Shenyang Normal University
Author Profile
Shuyan Cui
Shenyang Normal University
Author Profile
Abid Ali
University of Agriculture Faisalabad
Author Profile
Guo Zheng
Shenyang Normal University
Author Profile


Sex is one of the important factors affecting gut microbiota. As key predators in agro-forestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between female and male. However, how sex affects gut microbiota of spiders is still unclear. Therefore, in this study, the compositions and diversities of gut bacteria, based on bacterial 16S rRNA gene sequencing, were compared between female and male Pardosa astrigera. We found that bacterial richness indices (P < 0.05) in female were significantly lower than male, meanwhile, β-diversity showed significantly different between female and male (P < 0.05). The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteria) were significantly higher in female than male (P < 0.05). Whereas, the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria), Ruminococcus and Fusicatenibacter (all belong to Firmicutes), were significantly higher in male than female (P < 0.05). The results of PICRUSt2 showed that amino acid and lipid metabolisms were significantly higher in female than male (P < 0.05), whereas glycan biosynthesis and metabolism was significantly higher in male than female (P < 0.05). Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera. Male P. astrigera dispersed more widely than the female hence the male had a higher bacterial diversity. While the distinct differences of bacterial composition mainly due to their different nutritional and energy requirements.
17 Nov 2021Submitted to Ecology and Evolution
17 Nov 2021Submission Checks Completed
17 Nov 2021Assigned to Editor
01 Dec 2021Reviewer(s) Assigned
16 Dec 2021Review(s) Completed, Editorial Evaluation Pending
10 Feb 2022Editorial Decision: Revise Minor
08 Mar 20221st Revision Received
08 Mar 2022Submission Checks Completed
08 Mar 2022Assigned to Editor
08 Mar 2022Review(s) Completed, Editorial Evaluation Pending
28 Mar 2022Editorial Decision: Accept