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Purification of polyclonal Immunoglobulin G from human serum using LigaGuardTM and LigaTrapTM adsorbents
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  • Wenning Chu,
  • Hannah Reese,
  • Dipendra Bhandari,
  • Jae Sly,
  • Michael Crapanzano,
  • Stefano Menegatti
Wenning Chu
North Carolina State University
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Hannah Reese
North Carolina State University
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Dipendra Bhandari
LigaTrap Technologies LLC
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Jae Sly
LigaTrap Technologies LLC
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Michael Crapanzano
LigaTrap Technologies LLC
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Stefano Menegatti
North Carolina State University
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Abstract

This study presents the chromatographic purification of immunoglobulin G (IgG) from human plasma using a two-column process integrating the peptide-based adsorbents LigaGuardTM, which captures non-Ig plasma proteins in flow-through mode, and LigaTrapTM, which isolates IgG in bind-and-elute. Buffer composition and column loading were optimized for both adsorbents. Two process configurations were evaluated. In the first design, plasma was fed to a LigaGuardTM column to capture plasma proteins, the effluent was loaded on the LigaTrapTM column, and the bound IgG was eluted with 63.8% global recovery and 99.7% purity; in comparison, Protein G agarose afforded ~67% recovery and 97.2% purity. In the alternative design, the LigaGuardTM column was utilized to polish the LigaTrapTM elution stream, affording 82.3% global recovery and 98.8% purity. Collectively, these results demonstrate the potential of a fully chromatographic process for purifying polyclonal IgG from plasma feedstocks.

Peer review status:IN REVISION

25 Jun 2021Submitted to AIChE Journal
07 Jul 2021Submission Checks Completed
07 Jul 2021Assigned to Editor
11 Jul 2021Reviewer(s) Assigned
09 Aug 2021Editorial Decision: Revise Major
28 Aug 20211st Revision Received
31 Aug 2021Submission Checks Completed
31 Aug 2021Assigned to Editor
02 Sep 2021Reviewer(s) Assigned
21 Sep 2021Editorial Decision: Revise Minor